BIO RAD MRC 1024 CONFOCAL IMAGE ANALYSIS SYSTEM

BIO RAD MRC 1024 共焦图像分析系统

• 批准号: 2775664
• 负责人: RICHARD E COGGESHALL
• 金额: $ 10万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-19 至 2000-02-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2775664
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; confocal scanning microscopy; neurosciences

This revised proposal requests the purchase of a Bio-Rad MRC-1024 system to be used by 7 Faculty at the University of Texas Medical Branch, all with NIH-supported funding in the field of Neuroscience or Neuroendocrinology. The funded projects require: 1) an upright light- microscope assembly, 2) lasers with excitation in the 488nm, 568nm and 633nm laser excitation range (which allow imaging in green, yellow/red and far red, 3) ability to merge two fluorescent markers onto one image in three dimensions, 4) ability to superimpose fluorescent images onto black and white images of histological material 5) relatively user friendly software, 6) history of support from the vendor and 7) users within the Houston-Galveston community who could help form a users' support group. The Bio-Rad NRC-1024 system plus our local committee meets all these requirements. We emphasize that the proposal includes plans for scheduling, maintenance and upgrades as well as plans for technological development of applications such as stereology. Each of the funded research projects will be extended and expanded by this Bio-Rad system. In addition, one area where technological advances might be made by our group is in the wedding of this technology to stereological principles. Thus, acquisition of this technology should materially advance knowledge in fields of neuroanatomy and neuroendocrinology, with particular reference to basic pain mechanisms, and regulation of reproductive systems and also in applications where the combination of confocal and electron microscopic analyses become important.

该修订提案要求购买Bio-Rad MRC-1024系统，供德克萨斯大学医学分支的7个学院使用，所有这些学院都获得了NIH在神经科学或神经内分泌学领域的资助。 获得资助的项目需要：1）直立光学显微镜组件，2）在488 nm、568 nm和633 nm激光激发范围内激发的激光器（其允许以绿色、黄色/红色和远红色成像，3）将两种荧光标记物合并到三维图像上的能力，4）将荧光图像叠加到组织学材料的黑色和白色图像上的能力，5）相对用户友好的软件，6）来自供应商的支持历史和7）Houston-Galveston社区内可以帮助形成用户支持组的用户。 Bio-Rad NRC-1024系统加上我们的当地委员会满足所有这些要求。 我们强调，该提案包括时间安排、维护和升级计划以及立体学等应用的技术开发计划。每个受资助的研究项目都将通过Bio-Rad系统进行扩展和扩展。 此外，我们小组可能取得技术进步的一个领域是将这种技术与体视学原理结合起来。 因此，这项技术的收购应大大推进神经解剖学和神经内分泌学领域的知识，特别是基本的疼痛机制，生殖系统的调节，并在共聚焦和电子显微镜分析的组合成为重要的应用。