BIO RAD MRC 1024 CONFOCAL IMAGE ANALYSIS SYSTEM

这项修订后的提案要求购买Bio-Rad MRC-1024系统，供德克萨斯大学医学分部的7个学院使用，所有这些都是由NIH支持的神经科学或神经内分泌学领域的资金。获得资助的项目需要：1)一个直立的光学显微镜组件，2)488 nm、568 nm和633 nm激光激发范围内的激光(可以进行绿、黄/红和远红色成像)，3)能够将两个荧光标记合并到一个三维图像上，4)能够将荧光图像叠加到组织材料的黑白图像上，5)相对用户友好的软件，6)供应商的支持历史和7)休斯顿-加尔维斯顿社区内可以帮助形成用户支持小组的用户。Bio-Rad NRC-1024系统加上我们的地方委员会符合所有这些要求。我们强调，该提案包括调度、维护和升级计划以及立体学等应用程序的技术开发计划。每个资助的研究项目都将通过这个Bio-Rad系统进行延伸和扩展。此外，我们团队可能取得技术进步的一个领域是这项技术与体视学原理的结合。因此，这项技术的获得将大大促进神经解剖学和神经内分泌学领域的知识，特别是关于基本疼痛机制和生殖系统调节的知识，以及在结合共焦和电子显微镜分析变得重要的应用中的知识。

This revised proposal requests the purchase of a Bio-Rad MRC-1024 system to be used by 7 Faculty at the University of Texas Medical Branch, all with NIH-supported funding in the field of Neuroscience or Neuroendocrinology. The funded projects require: 1) an upright light- microscope assembly, 2) lasers with excitation in the 488nm, 568nm and 633nm laser excitation range (which allow imaging in green, yellow/red and far red, 3) ability to merge two fluorescent markers onto one image in three dimensions, 4) ability to superimpose fluorescent images onto black and white images of histological material 5) relatively user friendly software, 6) history of support from the vendor and 7) users within the Houston-Galveston community who could help form a users' support group. The Bio-Rad NRC-1024 system plus our local committee meets all these requirements. We emphasize that the proposal includes plans for scheduling, maintenance and upgrades as well as plans for technological development of applications such as stereology. Each of the funded research projects will be extended and expanded by this Bio-Rad system. In addition, one area where technological advances might be made by our group is in the wedding of this technology to stereological principles. Thus, acquisition of this technology should materially advance knowledge in fields of neuroanatomy and neuroendocrinology, with particular reference to basic pain mechanisms, and regulation of reproductive systems and also in applications where the combination of confocal and electron microscopic analyses become important.