CORE-- NEUROPATHOLOGY

核心——神经病理学

• 批准号: 6098330
• 负责人: WILLIAM ELLIS
• 金额: $ 31.39万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6098330
• 关键词: Alzheimer's disease; amyloid proteins; biomedical facility; brain disorder diagnosis; clinical research; dementia; disease /disorder classification; family genetics; genetic markers; histopathology; human genetic material tag; human subject; information dissemination; messenger RNA; neural degeneration; neuritic plaques; neuroanatomy; neurofibrillary tangles; neurons; neurotransmitters; postmortem; stainings; synapses; tissue resource /registry

The Neuropathology Core of the UC Davis Alzheimer's Disease Center (UCD ADC) has 3 major goals: (1) to obtain brain autopsies on ADC patients and controls, (2) to accurately diagnose all brain lesions encountered at autopsy in these individuals and transfer information about these diagnoses to the clinical core, and (3) to preserve and distribute autopsy brain tissues to research collaborators. This Core has developed a protocol in conjunction with the Clinical Core for recruitment of subjects to the brain donation program which relies upon ADC clinicians to approach patients or controls early on in the evaluation process. Once recruited, a number of methods have been developed for the distribution and tracking of signed autopsy consents and procedural information so that, at the time of death, the mechanism for activating the brain retrieval protocol is simple and straightforward. This protocol relies upon 24-hour a day on-call trained technicians who travel to the local mortuary (or other facility) to remove the brain. In most cases, half the brain is formalin fixed and the other half is coronally sectioned and frozen between dry ice. In special situations such as cerebrovascular disease, advance information is provided about lesion location so that the relevant area is formalin fixed. Provisions for very rapid brain removal and tissue processing are also made for some collaborators. Subsequent processing is performed in the Neuropathology Core Laboratory in Davis. Initially, 25 blocks of formalin fixed tissue will be obtained for paraffin embedment from multiple neocortical, limbic, diencephalic, basal ganglia, and brainstem regions. All sections will be stained with hematoxylin-eosin and a subgroup with Bielschowsky's sliver technique. Selected blocks will be stained with Luxol fast blue-cresyl violet to identify periventricular white matter degeneration, and with Congo red and beta amyloid immunostain to detect associated amyloid angiopathy. The blocks taken are compatible with those suggested by the Consortium to Establish a Registry for Alzheimer's Disease (CERAD). All tissue will be examined by a single neuropathologist, the Core PI, who will produce a standard gross and microscopic neuropathology report, and will quantitate neuritic plaques and neurofibrillary tangles. This method permits classification of all pathological diagnoses according to the suggested CERAD schema. Final reports are returned to the Clinical Core, where they are communicated to the patient's next of kin by ADC clinicians familiar with the case. Tissue and brain DNA is banked in the neuropathology laboratory. mRNA screening of frozen specimens will be used to assess the acceptability of tissue for molecular and biochemical studies. All data are logged into the Neuropathology Laboratory data base, which includes an inventory of all tissue, mode of processing and storage, pathological diagnosis, and autolysis interval. Quantitative data and final neuropathologic diagnoses according to CERAD criteria are recorded and entered into the centralized database, where it is readily linked to other data.

加州大学戴维斯分校阿尔茨海默病中心(UCD)的神经病理学核心 ADC)有三个主要目标：(1)获得ADC患者的脑部尸检和 对照，(2)准确诊断所有脑部病变 对这些人进行尸检，并转移关于这些人的信息 临床核心的诊断；(3)保存和分发尸检 脑组织给研究合作者。 这个核心已经与临床核心一起开发了一项协议 招募受试者参加大脑捐赠计划，该计划依赖于 要求ADC临床医生及早接触患者或对照 评估过程。一旦被招募，就有许多方法 为分发和跟踪签署的尸检同意书而开发的 程序性信息，以便在死亡时， 激活大脑检索协议是简单和直接的。 该协议依赖于一天24小时待命的训练有素的技术人员 去当地的太平间(或其他设施)摘除大脑。在……里面 大多数情况下，一半的大脑是福尔马林固定的，另一半是 在干冰之间被冠状切开和冻结。在特殊情况下 例如脑血管疾病，提供了关于 病变部位使相关区域福尔马林固定。条文 对于非常快速的大脑移除和组织处理，也为一些 合作者。 后续处理在神经病理学核心实验室进行 在戴维斯。最初，将获得25块福尔马林固定组织 石蜡包埋来自多个新皮质，边缘，间脑， 基底节和脑干区。所有部分都将被染上 苏木精-伊红和一个亚群的Bielschowsky的银条技术。 选定的块将用卢克索坚牢蓝-甲酚紫染成 鉴别脑室周围白质变性，并与刚果红和 β淀粉样蛋白免疫染色检测相关的淀粉样血管病变。这个 所采用的区块与财团建议的区块相兼容 建立阿尔茨海默病登记处(CERAD)。 所有组织都将由一名神经病理学家进行检查，核心PI是 将产生一份标准的大体和显微神经病理报告，以及 将量化神经炎斑块和神经原纤维缠结。这种方法 允许对所有病理诊断进行分类 建议的CERAD架构。 最终报告被退回到临床核心，它们在那里 由熟悉以下内容的ADC临床医生与患者的近亲沟通 这个案子。组织和脑DNA储存在神经病理学中 实验室。冰冻标本的mRNA筛选将用于评估 组织对分子和生物化学研究的可接受性。所有数据 登录到神经病理实验室数据库，其中包括 所有组织的库存、加工和储存方式、病理 诊断和自溶间期。量化数据和最终数据 根据CERAD标准记录神经病理诊断并 输入中央数据库，在那里它可以很容易地与其他 数据。