CORE-- NEUROPATHOLOGY

核心——神经病理学

• 批准号: 6295542
• 负责人: WILLIAM ELLIS
• 金额: $ 32.87万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295542
• 关键词: Alzheimer's disease; amyloid proteins; biomedical facility; brain disorder diagnosis; clinical research; dementia; disease /disorder classification; family genetics; genetic markers; histopathology; human genetic material tag; human subject; information dissemination; messenger RNA; neural degeneration; neuritic plaques; neuroanatomy; neurofibrillary tangles; neurons; neurotransmitters; postmortem; stainings; synapses; tissue resource /registry

The Neuropathology Core of the UC Davis Alzheimer's Disease Center (UCD ADC) has 3 major goals: (1) to obtain brain autopsies on ADC patients and controls, (2) to accurately diagnose all brain lesions encountered at autopsy in these individuals and transfer information about these diagnoses to the clinical core, and (3) to preserve and distribute autopsy brain tissues to research collaborators. This Core has developed a protocol in conjunction with the Clinical Core for recruitment of subjects to the brain donation program which relies upon ADC clinicians to approach patients or controls early on in the evaluation process. Once recruited, a number of methods have been developed for the distribution and tracking of signed autopsy consents and procedural information so that, at the time of death, the mechanism for activating the brain retrieval protocol is simple and straightforward. This protocol relies upon 24-hour a day on-call trained technicians who travel to the local mortuary (or other facility) to remove the brain. In most cases, half the brain is formalin fixed and the other half is coronally sectioned and frozen between dry ice. In special situations such as cerebrovascular disease, advance information is provided about lesion location so that the relevant area is formalin fixed. Provisions for very rapid brain removal and tissue processing are also made for some collaborators. Subsequent processing is performed in the Neuropathology Core Laboratory in Davis. Initially, 25 blocks of formalin fixed tissue will be obtained for paraffin embedment from multiple neocortical, limbic, diencephalic, basal ganglia, and brainstem regions. All sections will be stained with hematoxylin-eosin and a subgroup with Bielschowsky's sliver technique. Selected blocks will be stained with Luxol fast blue-cresyl violet to identify periventricular white matter degeneration, and with Congo red and beta amyloid immunostain to detect associated amyloid angiopathy. The blocks taken are compatible with those suggested by the Consortium to Establish a Registry for Alzheimer's Disease (CERAD). All tissue will be examined by a single neuropathologist, the Core PI, who will produce a standard gross and microscopic neuropathology report, and will quantitate neuritic plaques and neurofibrillary tangles. This method permits classification of all pathological diagnoses according to the suggested CERAD schema. Final reports are returned to the Clinical Core, where they are communicated to the patient's next of kin by ADC clinicians familiar with the case. Tissue and brain DNA is banked in the neuropathology laboratory. mRNA screening of frozen specimens will be used to assess the acceptability of tissue for molecular and biochemical studies. All data are logged into the Neuropathology Laboratory data base, which includes an inventory of all tissue, mode of processing and storage, pathological diagnosis, and autolysis interval. Quantitative data and final neuropathologic diagnoses according to CERAD criteria are recorded and entered into the centralized database, where it is readily linked to other data.

加州大学戴维斯分校阿尔茨海默病中心（UCD）的神经病理学核心 ADC）有3个主要目标：（1）获得ADC患者的脑尸检， 对照组，（2）准确诊断所有脑病变， 对这些人进行尸检， 诊断的临床核心，（3）保存和分发尸检 脑组织的研究合作者。 该核心与临床核心共同制定了一项方案 为大脑捐赠计划招募受试者， ADC临床医生在早期接触患者或对照组， 评估过程。 一旦被招募， 开发用于分发和跟踪已签署的尸检同意书， 程序信息，以便在死亡时， 激活大脑检索协议是简单而直接的。 该协议依赖于24小时待命的训练有素的技术人员， 前往当地太平间（或其他设施）取出大脑。 在 大多数情况下，一半的大脑是福尔马林固定的，另一半是 冠状切片并在干冰中冷冻。 处境特殊的 例如脑血管疾病，提供了关于 病变部位，使相关区域用福尔马林固定。 规定 用于非常快速的大脑切除和组织处理， 合作者 后续处理在神经病理学核心实验室进行 在戴维斯。 最初，将获得25块福尔马林固定的组织 用于从多个新皮层，边缘，间脑， 基底神经节和脑干区域。 所有切片将用 苏木精-伊红染色法和Bielschowsky银染法。 选择的组织块将用Luxol坚牢蓝-甲酚紫染色， 鉴别脑室周围白色物质变性，并用刚果红和 β淀粉样蛋白免疫染色检测相关的淀粉样血管病。 的 所采取的区块与联合体建议的区块一致， 建立阿尔茨海默病登记处（CERAD）。 所有组织将由一名神经病理学家（核心PI）进行检查， 将生成标准的大体和显微神经病理学报告， 将定量神经炎斑块和神经纤维缠结。 该方法 允许根据所有病理诊断的分类 建议CERAD模式。 最终报告将返回临床中心， 由ADC临床医生告知患者的近亲， 这个案子了 组织和大脑DNA储存在神经病理学中 实验室 冷冻标本的mRNA筛选将用于评估 用于分子和生物化学研究的组织的可接受性。 所有数据 被记录到神经病理学实验室数据库，其中包括一个 所有组织的库存、处理和储存模式、病理学 诊断和自溶间隔。 定量数据和最终 记录根据CERAD标准的神经病理学诊断， 输入中央数据库，在那里它很容易链接到其他 数据