ESTIMATION OF TWO PHOTON DOSE VOLUME BY NUMERICAL SIMULATION

我们的大多数基于MPM的研究涉及成像深在 活体厚样本，从孤立的神经节到活体 小鼠 我们还需要一个系统，其中笼状试剂和 可以进行神经递质激活和电生理学 同时，在成像。 需要一台正置显微镜， 该系统允许使用长工作距离、水 浸没光学 基于Olympus AX-70固定载物台的系统 正置显微镜正在建设中，将有助于电压 电流钳位、膜片钳、连续数据（图像） 采集到硬盘，以及用于多光子释放的设施。 还包括用于体内的生理监测仪器 哺乳动物的实验。 这个系统被用于我们的许多 目前的合作需要在生命系统中进行深度成像。

The majority of our MPM based research involves imaging deep in living thick samples, examples ranging from isolated ganglia to living mice. We also need a system in which both caged reagent and neurotransmitter activation and electrophysiology can be carried out simultaneously during imaging. An upright microscope is required for this system to permit the use of the long working distance, water immersion optics. A system based on a fixed-stage Olympus AX-70 upright microscope is being constructed, and will facilitate voltage and current clamping, patch clamping, continuous data (image) acquisition to hard disk, and facilities for multiphoton uncaging. Also included is physiological monitoring instrumentation for in vivo experiments with mammals. This system is being used in many of our current collaborations that require deep imaging in living systems.