CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES

抗 DNA 自身抗体活性位点的表征

• 批准号: 6206033
• 负责人: GREG WORKMAN
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206033
• 关键词: biological products; biomedical resource; proteins

Antifreeze proteins were found to lower the freezing point of water on physiological fluid. The aim of the study is to understand the mechanism of such transition. Binding of proteins on certain lattice positions of ice, a pattern will form. The pattern growth will be observed with the fluorescence microscope. At lower temperatures the antifreeze protein is incorporated inside the ice crystals. It has been suggested that the proteins are highly organized within the ice crystal. Fluorescence polarizafion/anisoscopy measurements should reveal this organization. Proteins will be labeled with a variety of fluorophores, such as fluorescamine, rhodamine, and fluorescein.

研究发现抗冻蛋白可以降低冰点 生理液上的水。 研究的目的是了解 这种转变的机制。 蛋白质与某些物质的结合 冰的格子位置，就会形成图案。 图案成长 将用荧光显微镜观察。 在较低的 抗冻蛋白融入冰中的温度 晶体。 有人认为这些蛋白质具有很高的 组织在冰晶内。 荧光 偏振/各向异性测量应该揭示这种组织。 蛋白质将被标记有各种荧光团，例如 荧光胺、罗丹明和荧光素。