CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
批准号:
6348068
负责人:
GREG WORKMAN
金额:
$0.05万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-08-01 至 2001-07-31
中文摘要
抗冻蛋白被发现可以降低冰点
生理液体上的水。这项研究的目的是了解
这种转变的机制。蛋白质与某些特定物质的结合
冰的晶格位置,会形成一种图案。模式增长
将用荧光显微镜观察。在较低位置
温度抗冻蛋白被结合到冰中
水晶。已有研究表明,这些蛋白质是高度
在冰晶中组织起来。荧光
偏振光/验光测量应该能揭示这种组织结构。
蛋白质将被各种荧光团标记,例如
荧光胺、罗丹明和荧光素。
英文摘要
Antifreeze proteins were found to lower the freezing point of
water on physiological fluid. The aim of the study is to understand
the mechanism of such transition. Binding of proteins on certain
lattice positions of ice, a pattern will form. The pattern growth
will be observed with the fluorescence microscope. At lower
temperatures the antifreeze protein is incorporated inside the ice
crystals. It has been suggested that the proteins are highly
organized within the ice crystal. Fluorescence
polarizafion/anisoscopy measurements should reveal this organization.
Proteins will be labeled with a variety of fluorophores, such as
fluorescamine, rhodamine, and fluorescein.
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CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
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批准号:6646001
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项目类别:
-
资助金额:$24.81万
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财政年份:2002
-
负责人:GREG WORKMAN
-
依托单位:
CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
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批准号:6206033
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项目类别:
-
资助金额:$0.05万
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财政年份:1999
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负责人:GREG WORKMAN
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依托单位:
CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
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批准号:6252724
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项目类别:
-
资助金额:$1.18万
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财政年份:1997
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负责人:GREG WORKMAN
-
依托单位:
CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES
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批准号:5224529
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:GREG WORKMAN
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依托单位:--
海外基金