CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES

抗冻蛋白被发现可以降低冰点 生理液体上的水。这项研究的目的是了解 这种转变的机制。蛋白质与某些特定物质的结合 冰的晶格位置，会形成一种图案。模式增长 将用荧光显微镜观察。在较低位置 温度抗冻蛋白被结合到冰中 水晶。已有研究表明，这些蛋白质是高度 在冰晶中组织起来。荧光 偏振光/验光测量应该能揭示这种组织结构。 蛋白质将被各种荧光团标记，例如 荧光胺、罗丹明和荧光素。

Antifreeze proteins were found to lower the freezing point of water on physiological fluid. The aim of the study is to understand the mechanism of such transition. Binding of proteins on certain lattice positions of ice, a pattern will form. The pattern growth will be observed with the fluorescence microscope. At lower temperatures the antifreeze protein is incorporated inside the ice crystals. It has been suggested that the proteins are highly organized within the ice crystal. Fluorescence polarizafion/anisoscopy measurements should reveal this organization. Proteins will be labeled with a variety of fluorophores, such as fluorescamine, rhodamine, and fluorescein.