CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES

抗 DNA 自身抗体活性位点的表征

• 批准号: 6348068
• 负责人: GREG WORKMAN
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6348068
• 关键词: biological products; biomedical resource; proteins

Antifreeze proteins were found to lower the freezing point of water on physiological fluid. The aim of the study is to understand the mechanism of such transition. Binding of proteins on certain lattice positions of ice, a pattern will form. The pattern growth will be observed with the fluorescence microscope. At lower temperatures the antifreeze protein is incorporated inside the ice crystals. It has been suggested that the proteins are highly organized within the ice crystal. Fluorescence polarizafion/anisoscopy measurements should reveal this organization. Proteins will be labeled with a variety of fluorophores, such as fluorescamine, rhodamine, and fluorescein.

防冻蛋白被发现可以降低冰点