CHARACTERIZATION OF ACTIVE SITES OF ANTI DNA AUTO ANTIBODIES

抗冻蛋白被发现可以降低 生理液体上的水。 这项研究的目的是了解 这种转变的机制。 蛋白质在某些 冰的格子位置，就会形成图案。 增长模式 将用荧光显微镜观察。 以较低 在低温下，抗冻蛋白质被结合到冰中， 晶体 有人认为，蛋白质是高度 组织在冰晶中。 荧光 偏振/各向异性测量应该揭示这种组织。 蛋白质将用各种荧光团标记，例如 荧光胺、罗丹明和荧光素。

Antifreeze proteins were found to lower the freezing point of water on physiological fluid. The aim of the study is to understand the mechanism of such transition. Binding of proteins on certain lattice positions of ice, a pattern will form. The pattern growth will be observed with the fluorescence microscope. At lower temperatures the antifreeze protein is incorporated inside the ice crystals. It has been suggested that the proteins are highly organized within the ice crystal. Fluorescence polarizafion/anisoscopy measurements should reveal this organization. Proteins will be labeled with a variety of fluorophores, such as fluorescamine, rhodamine, and fluorescein.