B-LACTAMASE AND DEVELOPMENT OF MYXOCOCCUS XANTHUS

本研究项目的目的是描述诱导物的作用。 黄色粘球菌发育过程中的β-内酰胺酶系统。 最近的证据表明，黄曲霉将遭受饥饿- 伴随着染色体诱导的独立产孢量 编码β-内酰胺酶(AmpC)。诱导型β-内酰胺酶的组成 肠道细菌中的系统已被证明是细胞壁所必需的 回收利用。因为黄曲霉的产孢量需要细胞壁 重塑，这导致了一种假设，即可诱导的组件 β-内酰胺酶系统是正常发育所必需的。以下是 将采取以下方法来检验这一假说：1)确定基因 参与聚合酶链式反应和转座子诱变β-内酰胺酶，2) 通过监测研究这些基因在发育过程中的调控 操纵子融合的表达，以及3)生化分析 Murein周转后细胞壁重塑中的基因产物 不同变异背景下的成分。

The goal of this research project is to describe the role of the inducible beta-lactamase system in the developmental program of Myxococcus xanthus. Recent evidence indicates that M.xanthus will undergo starvation- independent sporulation with concomitant induction of a chromosomally encoded beta-lactamase (ampC). Components of the inducible beta-lactamase system in the enterobacteria have been shown to be required for cell wall recycling. Because sporulation in M. xanthus requires cell wall remodeling, this leads to the hypothesis that components of the inducible beta-lactamase system are required for normal development. The following approach will be taken to test this hypothesis: 1) identify the genes involved in beta-lactamase induction by PCR and transposon mutagenesis, 2) examine regulation of these genes during development by monitoring expression of operon fusions, and 3) analyze biochemically the role of gene products in cell wall remodeling by following turnover of murein components in various mutant backgrounds.