The Evolutionary Dynamics of Genetic Conflict: the Origin, Maintenance and Loss of Paternal Genome Elimination.

遗传冲突的进化动力学：父系基因组消除的起源、维持和丧失。

• 批准号: NE/K009516/1
• 负责人: Laura Ross
• 金额: $ 67.25万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FK009516%2F1
• 关键词: Evolutionary; Dynamics; Genetic; Conflict; Origin

Reproduction across the tree of life: Why so many ways of doing it?During sexual reproduction, two unrelated individuals cooperate to achieve a common goal: pass on their genes to the next generation. This cooperation is however not without conflict, parents can fight over who raises the kids, and partners can cheat and mate with other mates. Despite these conflicts genes of the mother and father are, at least in most animals, equally represented in the offspring. This is, however, not always the case. In my research I study a group of insects whose reproduction is incredibly variable and where evolutionary innovations appear to have reduced the importance of males. For example in the citrus mealybug (a plant parasite) and the human body lice (our own parasite), males are still needed to fertilize females, but the female can eliminate his genes from her son. This unusual type of reproduction called "Paternal Genome Elimination" (PGE) is in the female's interest as her sons will always pass on her genes, instead of those of her partner. I use these two species to study how conflict between the sexes can have lead to the evolution of this strange reproductive behaviour. I aim to understand how males and females compete by studying the behavior of their genes within their offspring, using a range of state-of-the-art genetic techniques. In mealybugs we know that a father's genes are not simply eliminated from its sons sperm, but also prevented from being expressed. I aim to study the expression of paternal genes in males of both mealybugs and lice to understand if these genes might have the opportunity to fight against their elimination.The way a father's genes are eliminated from his sons is still unclear, although it seems to take place in the testis while sperm is produced. I aim to study the mechanism of elimination in lice by comparing the process of sperm production in males with and without PGE. Some of the finest minds in evolutionary biology have theorized about the evolution of PGE, yet to date, none of these theories have been tested empirically. Here I will use the human body louse, in which PGE has only recently been discovered to test these theories. I will use crosses between individuals with and without PGE to study how this trait is inherited and how it can spread though the population.In most species with PGE it seems that the mother's genes have won and ensured that they are the ones their sons will pass on to his children. This might not always be the case though. In the final project of this proposal I will study a clade of Australian insect, closely related to mealybugs. Previous research suggests that some species have lost PGE and might have reverted back to "normal" reproduction. I will study species across this clade to see how often PGE has been lost and which factors might be responsible.Taken together, these approaches will address the forces responsible for the evolution of an unusual reproductive system. This knowledge will help scientists understand why reproduction, a process so fundamental, is so variable across life.

生命之树的繁殖：为什么有这么多的方式？在有性生殖过程中，两个不相关的个体合作以实现一个共同的目标：将它们的基因传递给下一代。然而，这种合作并不是没有冲突的，父母可能会为谁抚养孩子而争吵，伴侣可能会欺骗和与其他伴侣交配。尽管存在这些冲突，至少在大多数动物中，父母的基因在后代中是平等的。然而，情况并非总是如此。在我的研究中，我研究了一群昆虫，它们的繁殖方式非常多变，进化的创新似乎降低了雄性的重要性。例如，在柑橘粉虱（一种植物寄生虫）和人体虱子（我们自己的寄生虫）中，雄性仍然需要使雌性受精，但雌性可以从她的儿子身上消除他的基因。这种不寻常的繁殖方式被称为“父系基因组消除”（PGE），这符合雌性的利益，因为她的儿子将永远传递她的基因，而不是她伴侣的基因。我用这两个物种来研究两性之间的冲突如何导致这种奇怪的繁殖行为的进化。我的目标是通过使用一系列最先进的基因技术，研究它们在后代中的基因行为，来了解雄性和雌性是如何竞争的。在粉蚧中，我们知道父亲的基因不仅从其儿子的精子中被消除，而且还被阻止表达。我的目的是研究父系基因在粉虱和虱子雄性中的表达，以了解这些基因是否有机会对抗它们的消灭。父亲的基因从儿子身上消失的方式尚不清楚，尽管它似乎发生在精子产生时的睾丸中。我的目的是通过比较有和没有PGE的雄性精子产生过程来研究虱子的消除机制。进化生物学中一些最优秀的人已经提出了关于PGE进化的理论，但到目前为止，这些理论都没有经过实证检验。在这里，我将使用人体虱子来测试这些理论，在人体虱子中，PGE最近才被发现。我将使用有和没有PGE的个体之间的杂交来研究这种特性是如何遗传的，以及它是如何在群体中传播的。在大多数具有PGE的物种中，似乎母亲的基因获胜了，并确保它们的儿子将把这些基因传递给他的孩子。但情况可能并非总是如此。在本次提案的最终项目中，我将研究澳大利亚昆虫的一个分支，与粉蚧密切相关。先前的研究表明，一些物种已经失去了PGE，可能已经恢复到“正常”繁殖。我将研究这个分支的物种，看看PGE丢失的频率，以及哪些因素可能是罪魁祸首。综上所述，这些方法将解决导致一个不寻常的生殖系统进化的力量。这一知识将帮助科学家理解为什么繁殖这个如此基本的过程在生命中如此多变。

项目成果

The unusual reproductive system of head and body lice (Pediculus humanus).

• DOI: 10.1111/mve.12287
• 发表时间: 2018-06
• 期刊: Medical and veterinary entomology
• 影响因子: 1.9
• 作者: DE LA Filia AG;Andrewes S;Clark JM;Ross L
• 通讯作者: Ross L

Sex determination: why so many ways of doing it?

• DOI: 10.1371/journal.pbio.1001899
• 发表时间: 2014-07
• 期刊: PLoS biology
• 影响因子: 9.8
• 作者: Bachtrog D;Mank JE;Peichel CL;Kirkpatrick M;Otto SP;Ashman TL;Hahn MW;Kitano J;Mayrose I;Ming R;Perrin N;Ross L;Valenzuela N;Vamosi JC;Tree of Sex Consortium
• 通讯作者: Tree of Sex Consortium

The evolutionary dynamics of haplodiploidy: Genome architecture and haploid viability.

• DOI: 10.1111/evo.12792
• 发表时间: 2015-11
• 期刊: Evolution; international journal of organic evolution
• 作者: Blackmon H;Hardy NB;Ross L
• 通讯作者: Ross L

Males That Silence Their Father's Genes: Genomic Imprinting of a Complete Haploid Genome.

• DOI: 10.1093/molbev/msab052
• 发表时间: 2021-05-19
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: de la Filia AG;Mongue AJ;Dorrens J;Lemon H;Laetsch DR;Ross L
• 通讯作者: Ross L