PHOSPHOLIPASE A1

磷脂酶A1

• 批准号: 6116299
• 负责人: John A. Glomset
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116299
• 关键词: Mammalia; Primates; human tissue; lipids; nervous system

We previously identified a phosphatidic-acid-preferring phospholipase A1, which was present in high amounts in the mature testis and brain. Its special properties and distribution suggested that it could be playing a hitherto undescribed role in cell-signaling pathways. We therefore purified the enzyme, cloned and sequenced its cDNA, and examined both the distribution of the enzyme's messenger RNA and the distribution of the enzyme protein in primate tissues. We identified messenger RNA molecules of several different sizes and showed that they were distributed differently in different tissues and different regions of the brain. We also identified proteins of different sizes, which cross-reacted with antibodies to the phospholipase A1, and showed that they too were distributed differently in different tissues and different regions of the brain. The combined results of these and other experiments suggest that primate tissues may contain a family of proteins that are related to the enzyme. We are using techniques of molecular biology to investigate this possibility, and we hope to characterize the presumptive family of proteins in preparation for more detailed messenger RNA and protein mapping experiments in macaques.

我们以前确定了磷脂酸偏好磷脂酶A1，这是目前在成熟的睾丸和大脑中的高金额。 它的特殊性质和分布表明，它可能在细胞信号通路中发挥迄今未描述的作用。 因此，我们纯化了这种酶，克隆并测序了它的cDNA，并检查了这种酶的信使RNA的分布和这种酶蛋白在灵长类动物组织中的分布。 我们鉴定了几种不同大小的信使RNA分子，并表明它们在大脑的不同组织和不同区域中分布不同。 我们还鉴定了不同大小的蛋白质，这些蛋白质与磷脂酶A1的抗体发生交叉反应，并表明它们在大脑的不同组织和不同区域中的分布也不同。 这些和其他实验的综合结果表明，灵长类动物组织可能含有一个与酶相关的蛋白质家族。 我们正在使用分子生物学技术来研究这种可能性，我们希望能够表征假定的蛋白质家族，为猕猴中更详细的信使RNA和蛋白质作图实验做准备。