PHOSPHOLIPASE A1

磷脂酶A1

• 批准号: 6219725
• 负责人: John A. Glomset
• 金额: $ 7.46万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6219725
• 关键词: Mammalia; human tissue; lipids; nervous system

We previously purified a soluble phosphatidic acid-preferring phospholipase A1 from bovine testis and cloned and sequenced its cDNA. This enzyme could potentially play an important role in phosphatidic acid-dependent cell signaling, but this role remains to be defined. Studies of the distribution of the enzyme's mRNA in human tissues demonstrated several reactive components, which were distributed differently in the testis, brain, and other tissues. Studies with antibodies to the bovine enzyme demonstrated cross-reactive material in macaque tissues including the testis. The testis enzyme could be precipitated from the high-speed supernatant of macaque testis homogenates with antibodies to the protein kinase, CK2. Furthermore, a recombinant form of the alpha subunit of CK2 could catalyze the phosphorylation of both native and recombinant forms of the phospholipase A1; and recombinant forms of the alpha subunit of CK2 and the phospholipase could form a tightly associa ted comple x. These results raise the novel possibility that CK2 may in some way regulate the function of the phospholipase, and attempts to test this possibility are currently underway. In addition, in situ hybridization and immunocytochemical studies are planned to examine the possibility that the mRNAs and proteins that correspond to CK2 and the phospholipase may be coexpressed. Another finding of potential interest is that material that cross-reacts with antibodies to the phospholipase is present in spermatozoa from the cauda epididymis. This material is of relatively low molecular mass (approximately 30 KDa) and is tightly associated with the tail. We are currently attempting to isolate amounts of this material sufficient for sequence analysis by mass spectroscopy. FUNDING NIH grant RR00166 and the Howard Hughes Medical Institute. Hollenback, D. and Glomset, J.A. On the relation between a stearoyl-specific transacylase from bovine testis membranes and a co-purifying acyltransferase. Biochemistry 37 363-376, 1998. Higgs, H.N., Han, M.H., Johnson, G.E., and Glomset, J.A. Cloning of a phosphatidic acid-preferring phospholipase A1 from bovine testis. J. Biol. Chem. 273:5468-5477, 1998. [This project was also reported in the Neuroscience Core section.]

我们先前纯化了一种可溶性磷脂酸偏好 从牛睾丸中分离到磷脂酶A1基因，克隆了其cDNA并进行了序列测定。 这种酶可能在磷脂合成中发挥重要作用。 酸依赖性细胞信号传导，但这种作用仍有待确定。 酶mRNA在人体组织中分布的研究 演示了几个反应组件，分布在 在睾丸、大脑和其他组织中的作用不同。 外贸 牛酶的抗体显示交叉反应物质 包括睾丸在内的猕猴组织中。 睾丸酶可能是 从猕猴睾丸的高速上清液中沉淀 匀浆与抗体的蛋白激酶，CK 2。 此外，委员会认为， CK 2 α亚基的重组形式可以催化 天然和重组形式的磷酸化 磷脂酶A1;以及CK 2 α亚基的重组形式 磷脂酶能形成紧密结合的复合物。这些 结果提出了新的可能性，CK 2可能以某种方式调节 磷脂酶的功能，并试图测试这一点， 可能性目前正在进行中。 此外，在现场 计划进行杂交和免疫细胞化学研究， 与CK 2和CK 3相对应的mRNA和蛋白质 磷脂酶可以共表达。 另一个潜在的发现 令人感兴趣是与抗 磷脂酶存在于来自附睾尾部的精子中。 该材料具有相对低的分子量（约30 它与尾巴紧密相连。 我们目前正在 试图分离出足够数量的这种物质 通过质谱分析。 资助NIH赠款RR 00166和 霍华德休斯医学研究所。 Hollenback，D.和Glomset，J.A.对 牛硬脂酰转酰酶 睾丸膜和共纯化酰基转移酶。 生物化学37 363-376，1998年。 希格斯，H.N.，Han，M.H.，约翰逊，通用电气，和格洛姆塞特 J.A.嗜磷脂酸磷脂酶A1基因的克隆 牛睾丸 J. Biol. Chem. 273：5468-5477，1998. [This项目 在神经科学核心部分也有报道。