ARACHIDONOYL DIACYLGLYCEROL KINASE

花生四烯酰二酰甘油激酶

• 批准号: 6116297
• 负责人: John A. Glomset
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116297
• 关键词: Primates; animal tissue; biological products; enzymes; human tissue; lipids; nervous system

Several years ago we discovered a new form of diacylglycerol kinase that preferentially catalyses the phosphorylation of diacylglycerol molecular species that contain esterified arachidonic acid. We also showed that high amounts of the enzyme are present in the testis and brain of the mature baboon. Since then we have been exploring the possibility that the enzyme may play a role in cell signaling processes that involve inositol-containing phophoglycerides. We have shown that the enzyme is present in preparations of synaptosomes and have also shown that the enzyme is present in very high concentrations in the macaque retina. This latter finding is of particular interest because it raises the possibility that phosphatidylinositol-dependent cell signaling may play a role in the visual process. What is needed now is to localize the enzyme in specific cells and cell membranes of the retina, and we will need specific antibodies for this immunochemical localization work. We have prepared antibodies directed to specific regions of the enzyme's sequence, and have used these antibodies in Western blotting experiments. But we have not yet succeeded in using them for immunochemical localization work. It may be that we will have to prepare monoclonal antibodies to the enzyme in the future.

几年前，我们发现了一种新形式的二酰基甘油激酶，它优先催化含有酯化花生四烯酸的二酰基甘油分子的磷酸化。我们还发现，在成熟狒狒的睾丸和大脑中存在大量的这种酶。从那时起，我们一直在探索这种酶可能在涉及含肌醇的磷酸甘油酯的细胞信号传导过程中发挥作用的可能性。我们已经证明这种酶存在于突触体的制备中，也表明这种酶在猕猴的视网膜中浓度非常高。后一项发现特别有趣，因为它提出了磷脂酰肌醇依赖性细胞信号传导可能在视觉过程中发挥作用的可能性。现在需要的是将酶定位在视网膜的特定细胞和细胞膜上，我们将需要特异性抗体来进行免疫化学定位工作。我们已经制备了针对酶序列特定区域的抗体，并在Western blotting实验中使用了这些抗体。但我们尚未成功地将它们用于免疫化学定位工作。在未来，我们可能需要制备针对该酶的单克隆抗体。