SENTINEL SPECIES--XENOBIOTICS, TOXICITY, AND REPRODUCTION

适当选择污染的监控物种和生物标志物， 结合生殖潜力和发育的评估， 提供对人类生殖的毒性影响的早期预警。 在这 项目我们建议研究地下水污染物的影响， 奥蒂斯空军基地超级基金网站（科德角）对三种生物存在于 在地下水的路径淡水（阿舒梅特池塘）的身体 羽毛。 这个池塘和其他三个池塘呈现出一种梯度， 与烟羽的距离和潜在的毒性。 被选中的“哨兵” （a）Anodonta cygnea，淡水贻贝，底栖物种。 (b)云斑叉尾鱼，棕色的牛头鲶鱼，一种海底生物。 (c)彩龟是一种远洋物种， 在沉积物中过冬 在具体目标1中，我们将评估区域 附近四个选定池塘中生物的毒素水平差异 阿舒梅特山谷的烟羽 在具体目标2中，我们将验证毒性 和鉴别（毒性鉴别评价，TIE） 阿舒梅特河谷地下沃茨水中含有的化学剂并进行评估 在地下水与地下水相互作用之前， 地表水 在具体目标3中，我们将评估肝脏效应（CYP 1A， CYP 4A、GST活性和卵黄蛋白原合成）对地下水的响应 在实验室中在从两个“干净的”脑桥收集的动物中（Long， Grews）。 在具体目标4中，我们将使用相同的 参数，在动物从四个相邻的池塘， 与地下水羽流相互作用的可能性不同。 在 具体目标5，使用相同的动物（具体目标4），我们的主要目标 是确定哪些生物效应与P450相关， 卵黄蛋白原生物标志物，使用内分泌/生殖参数。 这些 研究旨在确定这些广泛分布的物种， 预测潜在污染和对人类生殖影响的有用指标。

Appropriate selection of sentinel species and biomarkers of pollution, coupled with assessment of reproductive potential and development may provide an early warning of toxic impact on human reproduction. In this project we propose to study the impact of ground water pollutants from the Otis Air Force Base Superfund site (Cape Cod) on three organisms present in the body of fresh water (Ashumet Pond) in the path of the ground water plume. This and three other ponds present a gradient with regard to distance from the plume and potential toxicity. The chosen "sentinel species are (a) Anodonta cygnea, the fresh water mussel, a benthic species. (b) Ictalurus nebulosus, the brown bullhead catfish, a bottom dweller. (c) Chrysemys picta, the painted turtle, a pelagic species which overwinters in the sediment. In Specific Aim 1, we will assess regional differences in toxin level in organisms present in four selected ponds near the Ashumet Valley plume. In Specific Aim 2, we will verify the toxicity and identity (Toxicity Identification Evaluations, TIE's) of he putative chemical agents contained within Ashumet Valley ground waters and evaluate its biological activity before interaction of the ground water with the surface water. In Specific Aim 3, we will assess hepatic effects (CYP1A, CYP4A, GST activity and vitellogenin synthesis) in response to ground water in the laboratory in animals harvested from the two "clean" pons (Long, Grews). In Specific Aim 4, we will assess hepatic effects, using the same parameters, in animals taken from the four neighboring ponds with differing potentials for interaction with the ground water plume. In Specific Aim 5, using the same animals (Specific Aim 4) our major objective is to establish what biological effects are correlated with P450 and vitellogenin biomarkers, using endocrine/reproductive parameters. These studies are intended to establish these widely distributed species as useful predictors of potential pollution and impact on human reproduction.