SENTINEL SPECIES--XENOBIOTICS, TOXICITY, AND REPRODUCTION

适当选择污染的前哨物种和生物标志物， 再加上对生殖潜力和发育的评估可能 对有毒物质对人类生殖的影响提供早期预警。在这 我们建议研究地下水污染物对 奥的斯空军基地超级基金网站(科德角)关于存在于 地下水路径上的淡水水体(Ashumet池塘) 羽毛。这个池塘和其他三个池塘形成了一个坡度 与烟羽的距离和潜在的毒性。被选中的“哨兵” (A)无齿淡水贻贝，一种底栖生物。 (B)金枪鱼，一种生活在底栖动物的褐头叉尾叉尾鱼。 (C)彩龟，一种远洋物种， 在沉积物中越冬。在具体目标1中，我们将评估区域 近海四个池塘中生物毒素水平的差异 阿舒米特山谷的羽流。在具体目标2中，我们将验证其毒性 和身份(毒性鉴定评估，TIE‘s) 阿舒米特山谷地下水中含有的化学制剂和评估 地下水与地下水相互作用前的生物活性 地表水。在具体目标3中，我们将评估肝脏效应(CYP1A， CyP4A、GST活性和卵黄蛋白原合成)对地下水的响应 在实验室里，动物从两个“干净”的脑桥(长， Grews)。在特定的目标4中，我们将使用相同的 参数，从邻近的四个池塘中取出的动物中 与地下水羽流相互作用的不同位势。在……里面 具体目标5，使用相同的动物(具体目标4)我们的主要目标 是确定哪些生物学效应与P450和 卵黄蛋白原生物标志物，使用内分泌/生殖参数。这些 研究旨在将这些分布广泛的物种确定为 对潜在污染和对人类生殖的影响的有用预测。

Appropriate selection of sentinel species and biomarkers of pollution, coupled with assessment of reproductive potential and development may provide an early warning of toxic impact on human reproduction. In this project we propose to study the impact of ground water pollutants from the Otis Air Force Base Superfund site (Cape Cod) on three organisms present in the body of fresh water (Ashumet Pond) in the path of the ground water plume. This and three other ponds present a gradient with regard to distance from the plume and potential toxicity. The chosen "sentinel species are (a) Anodonta cygnea, the fresh water mussel, a benthic species. (b) Ictalurus nebulosus, the brown bullhead catfish, a bottom dweller. (c) Chrysemys picta, the painted turtle, a pelagic species which overwinters in the sediment. In Specific Aim 1, we will assess regional differences in toxin level in organisms present in four selected ponds near the Ashumet Valley plume. In Specific Aim 2, we will verify the toxicity and identity (Toxicity Identification Evaluations, TIE's) of he putative chemical agents contained within Ashumet Valley ground waters and evaluate its biological activity before interaction of the ground water with the surface water. In Specific Aim 3, we will assess hepatic effects (CYP1A, CYP4A, GST activity and vitellogenin synthesis) in response to ground water in the laboratory in animals harvested from the two "clean" pons (Long, Grews). In Specific Aim 4, we will assess hepatic effects, using the same parameters, in animals taken from the four neighboring ponds with differing potentials for interaction with the ground water plume. In Specific Aim 5, using the same animals (Specific Aim 4) our major objective is to establish what biological effects are correlated with P450 and vitellogenin biomarkers, using endocrine/reproductive parameters. These studies are intended to establish these widely distributed species as useful predictors of potential pollution and impact on human reproduction.