Core--Cytochemistry

细胞技术支持核心是对现有实验室核心的重大改进，该核心以高效和具有成本效益的方式为组织和沉积物中的痕量金属和挥发性有机物提供常规样本收集、准备和化学分析。随着BU SBRP内研究项目的发展和成熟，很明显，目前需要一个共享的设施来执行九个项目中的六个项目使用的各种细胞技术。这些细胞化学技术主要是标准组织学、免疫细胞化学、原位杂交和原位聚合酶链式反应。拟议的设施将在现有的电子显微镜和共焦显微镜设施内进行，并将配备一名专门的实验室技术员，与目前负责共焦显微镜的技术员一起工作。SBRP项目的更改将仅针对专门的，而不是普通的化学品和试剂。

The Cytotechniques Support Core represents a substantial modification of the existing Laboratory Core, which provided routine sample collection, preparation, chemical analysis for trace metals and volatile organics in tissues and sediments in an efficient and cost-effective fashion. With the evolution and maturation of the research program within the BU SBRP it has become evident that a current need is for a shared facility to perform a variety of cytotechniques used by six of nine projects. These cytochemical techniques are primarily standard tissue histology, immunocytochemistry, in situ hybridization and in situ PCR. The proposed facility with within an existing Electron Microscopy and Confocal Microscope facility, and will be staffed by a dedicated laboratory technician who will work with the current technician in charge of confocal microscopy. Changes to SBRP project will be made only for specialized, as opposed to general chemicals and reagents.