Core--Cytochemistry

细胞技术支持核心是对现有实验室核心的重大改进，现有实验室核心以高效和经济的方式提供常规样品收集，制备，组织和沉积物中痕量金属和挥发性有机物的化学分析。随着BU SBRP研究项目的发展和成熟，很明显，目前需要一个共享的设施来执行9个项目中的6个项目使用的各种细胞技术。这些细胞化学技术主要是标准组织组织学、免疫细胞化学、原位杂交和原位PCR。拟议的设施将在现有的电子显微镜和共聚焦显微镜设施内，并将配备一名专门的实验室技术人员，他将与目前负责共聚焦显微镜的技术人员一起工作。对SBRP项目的更改将只针对专业化学品和试剂，而不是一般化学品和试剂。

The Cytotechniques Support Core represents a substantial modification of the existing Laboratory Core, which provided routine sample collection, preparation, chemical analysis for trace metals and volatile organics in tissues and sediments in an efficient and cost-effective fashion. With the evolution and maturation of the research program within the BU SBRP it has become evident that a current need is for a shared facility to perform a variety of cytotechniques used by six of nine projects. These cytochemical techniques are primarily standard tissue histology, immunocytochemistry, in situ hybridization and in situ PCR. The proposed facility with within an existing Electron Microscopy and Confocal Microscope facility, and will be staffed by a dedicated laboratory technician who will work with the current technician in charge of confocal microscopy. Changes to SBRP project will be made only for specialized, as opposed to general chemicals and reagents.