Core--Cytochemistry

细胞技术支持核心是对现有实验室核心的重大修改，该实验室核心以有效和具有成本效益的方式提供组织和沉积物中痕量金属和挥发性有机物的常规样品收集、制备和化学分析。随着BU SBRP研究计划的发展和成熟，很明显，目前需要一个共享设施来执行九个项目中的六个项目所使用的各种细胞技术。这些细胞化学技术主要是标准组织组织学、免疫细胞化学、原位杂交和原位PCR。拟建机构位于现有电子显微镜和共聚焦显微镜机构内，将配备一名专门的实验室技术人员，该技术人员将与负责共聚焦显微镜的现有技术人员一起工作。SBRP项目的变更仅适用于专用化学品和试剂，而非通用化学品和试剂。

The Cytotechniques Support Core represents a substantial modification of the existing Laboratory Core, which provided routine sample collection, preparation, chemical analysis for trace metals and volatile organics in tissues and sediments in an efficient and cost-effective fashion. With the evolution and maturation of the research program within the BU SBRP it has become evident that a current need is for a shared facility to perform a variety of cytotechniques used by six of nine projects. These cytochemical techniques are primarily standard tissue histology, immunocytochemistry, in situ hybridization and in situ PCR. The proposed facility with within an existing Electron Microscopy and Confocal Microscope facility, and will be staffed by a dedicated laboratory technician who will work with the current technician in charge of confocal microscopy. Changes to SBRP project will be made only for specialized, as opposed to general chemicals and reagents.