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STRUCTURE OF MALTOSE TRANSPORTER: E COLI MEMBRANE ATP BINDING SUBUNIT

STRUCTURE OF MALTOSE TRANSPORTER: E COLI MEMBRANE ATP BINDING SUBUNIT
麦芽糖转运蛋白的结构:大肠杆菌膜 ATP 结合亚基
批准号:
6120587
负责人:
HOWARD A SHUMAN
金额:
$0.6万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-04-01 至 2000-03-31

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中文摘要
翻译
他们相信他们已经用DNA构建了一个共价晶格 齐聚物。建筑的基本单位是一个 反平行DNA双交换分子。这些都被结扎起来了 变成了他们认为是关于床单的东西。我第一次尝试 想象一下,在STEM中发现的壁到壁蛋白酶K具有 被用来阻止结扎。第二次尝试发现 缓冲区控制相当脏,因此无法区分 从样本中,尽管它现在不含外来蛋白质。在……里面 在两次尝试中,都可以找到一些诱人的领域。进一步工作 等待进一步的净化。此外,他们还试图 将其中一个组分低聚物的末端标上十年期。 这应该更好地在STEM fl=他们的早期可视化 企图使用胶体金。
英文摘要
They believe they have built a 2-1) covalent lattice from DNA oligomers. The fundamental unit of the construction is an antiparallel DNA double crossover molecule. These have been ligated into what they believe are about I g sheets. Ile first attempt to visualize this in the STEM found wall-to-wall proteinase K which had been used to stop the ligation. The second attempt found that the buffer control was rather dirty and consequently indistinguishable from the sample, though it was now free of extraneous protein. In both tries, some tantalizing areas could be found. Further work awaits additional purification. In addition, they were trying to label the end of one of the component oligoiners with undecagold. This should be much better to visualize in the STEM fl= their early attempts using colloidal gold.
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