GAMMA TUBULIN RING COMPLEX STRUCTURE: CENTROMERIC MICROTUBLES:DROSOPHILA EMBRYOS

伽玛微管蛋白环复合体结构：着丝粒微管：果蝇胚胎

• 批准号: 6120594
• 负责人: Yixian Zheng
• 金额: $ 1.18万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120594
• 关键词: biomedical resource; genetics; growth /development; human tissue; nucleic acids; plants

The origin recognition complex (ORQ in yeast is composed of six subunits. They are attemptmg to crystallize it to determme its structure by x-ray crystallography. Meanwhile, they would like to determine the stoichiometry of the complex using-the STEM. Preliminary results have been somewhat encouragmg, in that while the complex seems:to fall apart on the carbon support film, it seems to be fairly well-preserved by a brief glutaraldehyde fixation. Both the ORC alone and the ORC plus DNA will be looked at

酵母中的起源识别复合物（origin recognition complex，ORQ）由六个 亚单位。 他们不愿意使它结晶， 由X射线晶体学构造。 与此同时，他们希望 使用STEM确定络合物的化学计量。 初步结果有些令人不安，因为 复杂的似乎：在碳支撑膜上分崩离析，它似乎是 通过短暂的戊二醛固定保存得相当好。 两者 将研究单独的ORC和ORC加DNA