Mechanisms of chromosome segregation and mitotic timing
Mechanisms of chromosome segregation and mitotic timing
批准号:
9043909
负责人:
Yixian Zheng
金额:
$37.41万
依托单位国家:
美国
项目类别:
财政年份:
2015
资助国家:
美国
项目状态:
已结题
起止时间:
2015-04-01 至 2019-01-31
关键词:
AnaphaseBehaviorBindingCell ProliferationChromosome SegregationChromosomesComplexEnsureGrantHandHealthKinetochoresLamin Type BMediatingMetaphaseMetaphase PlateMicrotubulesMitosisMitoticMitotic CheckpointModelingMolecularMolecular ChaperonesProcessPrometaphaseProteinsProteomeRegulationRoleSideSignal TransductionTimeXenopuseggprotein functionscreeningtherapeutic targetubiquitin-protein ligase
中文摘要
描述(由申请人提供):本提案旨在研究动粒蛋白捕获微管以确保染色体分离均等和有丝分裂及时进展的机制。通过分析纺锤体相关的含层粘连蛋白B的网络,我们以前称之为纺锤体基质,我们已经做出了一些令人兴奋的观察,这表明层粘连蛋白B网络的组分与微管一起作为动粒蛋白如Bub3的伴侣蛋白发挥作用。这些分子伴侣是着丝粒蛋白调节适当的微管和着丝粒相互作用和及时的有丝分裂进程所必需的。我们建议剖析这些分子伴侣在有丝分裂中发挥作用的分子机制。具体来说,在目标1中,我们将剖析BuGZ和微管和/或EB 1之间的相互作用是否以及如何需要Bub3动粒加载和染色体比对。在目的2中,我们将研究RanGTP对BuGZ和Bub3的不稳定是否有助于纺锤体组装检查点(SAC)的沉默。在目标3中,我们提出研究介导末端着丝粒-MT附着和SAC沉默的分子机制。
英文摘要
DESCRIPTION (provided by applicant): This proposal aims to study the mechanism by which kinetochore proteins capture microtubules to ensure equal chromosome segregation and timely progression through mitosis. Through analyzing the spindle-associated lamin-B containing network, which we had referred to as the spindle matrix previously, we have made a number of exciting observations, which suggest that component(s) of the lamin-B network functions together with microtubules as chaperones for kinetochore proteins such as Bub3. These chaperones are required for kinetochore proteins to regulate proper microtubule and kinetochore interactions and timely mitosis progression. We propose to dissect the molecular mechanism by which these chaperones function in mitosis. Specifically, in Aim 1 we will dissect whether and how the interaction between BuGZ and microtubules and/or EB1 is required for Bub3 kinetochore loading and chromosome alignment. In Aim 2 we will study whether the destabilization of BuGZ and Bub3 by RanGTP contributes to silencing of the spindle assembly checkpoint (SAC). In Aim 3, we propose to study the molecular mechanisms that mediate end-on kinetochore-MT attachment and SAC silencing.
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会议论文
The Mechanism of Spindle Assembly and Chromosome Alignment
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批准号:10246849
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项目类别:
-
资助金额:$35.54万
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财政年份:2018
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负责人:Yixian Zheng
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依托单位:
Mechanisms of chromosome segregation and mitotic timing
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批准号:9205525
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项目类别:
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资助金额:$38.94万
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财政年份:2015
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负责人:Yixian Zheng
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依托单位:
The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
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批准号:8209221
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项目类别:
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资助金额:$18.53万
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财政年份:2011
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负责人:Yixian Zheng
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依托单位:
The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
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批准号:8033327
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项目类别:
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资助金额:$22.78万
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财政年份:2011
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负责人:Yixian Zheng
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依托单位:
XENOPUS SPINDLE MATRIX PROTEOME
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批准号:7957759
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项目类别:
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资助金额:$0.33万
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财政年份:2009
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负责人:Yixian Zheng
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依托单位:
XENOPUS SPINDLE MATRIX PROTEOME
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批准号:7723678
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项目类别:
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资助金额:$0.08万
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财政年份:2008
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负责人:Yixian Zheng
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依托单位:
, STRUCTURE OF Y TUBULIN RING COMPLEX
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批准号:6444671
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项目类别:
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资助金额:$29.31万
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财政年份:2001
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负责人:Yixian Zheng
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依托单位:
, STRUCTURE OF Y TUBULIN RING COMPLEX
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批准号:6308919
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项目类别:
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资助金额:$0.97万
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财政年份:2000
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负责人:Yixian Zheng
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依托单位:
GAMMA TUBULIN RING COMPLEX STRUCTURE: CENTROMERIC MICROTUBLES:DROSOPHILA EMBRYOS
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批准号:6120594
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项目类别:
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资助金额:$1.18万
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财政年份:1999
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负责人:Yixian Zheng
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依托单位:
, STRUCTURE OF Y TUBULIN RING COMPLEX
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批准号:6281368
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项目类别:
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资助金额:$0.32万
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财政年份:1998
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负责人:Yixian Zheng
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依托单位:
Gamma tubulin ring complex in microtubule nucleation
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批准号:6653829
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项目类别:
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资助金额:$18.32万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
Regulation of Microtubule Nucleation During Spindle Assembly
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批准号:7923861
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项目类别:
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资助金额:$15.44万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
The function and assembly of the lamin-B spindle sheath
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批准号:8371730
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项目类别:
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资助金额:$35.1万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
The function and assembly of the lamin-B spindle sheath
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批准号:8538992
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项目类别:
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资助金额:$33.87万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
Gamma tubulin ring complex in microtubule nucleation
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批准号:6794658
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项目类别:
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资助金额:$15.6万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
Gamma tubulin ring complex in microtubule nucleation
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批准号:6543794
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项目类别:
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资助金额:$19.49万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
Regulation of Microtubule Nucleation During Spindle Assembly
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批准号:7688608
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项目类别:
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资助金额:$15.6万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
The function and assembly of the lamin-B spindle sheath
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批准号:8727573
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项目类别:
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资助金额:$35.1万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
Gamma tubulin ring complex in microtubule nucleation
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批准号:6944054
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项目类别:
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资助金额:$14.67万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
DROSOPHILA & TUBULIN RING COMPLEX (ANDTURC)
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批准号:2771099
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项目类别:
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资助金额:$18.7万
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财政年份:1997
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负责人:Yixian Zheng
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依托单位:
国内基金
海外基金
greenwashing behavior in China:Basedon an integrated view of reconfiguration of environmental authority and decoupling logic
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项目类别:外国学者研究基金项目
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批准年份:2024
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负责人:YU BYUNGJUN
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依托单位:
Incentive and governance schenism study of corporate green washing behavior in China: Based on an integiated view of econfiguration of environmental authority and decoupling logic
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项目类别:外国学者研究基金项目
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批准年份:2024
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负责人:YU BYUNGJUN
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依托单位: