DROSOPHILA & TUBULIN RING COMPLEX (ANDTURC)

果蝇

• 批准号: 2771099
• 负责人: Yixian Zheng
• 金额: $ 18.7万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2771099
• 关键词: cell cycle; centrosome; electron microscopy; microtubule associated protein; microtubules; molecular cloning; protein structure function

DESCRIPTION: The long-term goal of this research is to understand how centrosomes in animal cells nucleate microtubules and how this nucleating activity is regulated by cell-cycle and developmental processes. The protein complex to be used in this study is the recently identified gamma-tubulin ring complex (gamma-TuRC) which can nucleate and bind to the minus ends of tits nucleated microtubules in vitro. Based on a number of lines of evidence it is hypothesized that the gamma-TuRC is the major microtubule nucleator at the centrosomes. The gamma-TuRC consists of gamma tubulin and at least five currently unidentified proteins (p183, p151, p98, p82 and p75s). The four aims of this proposal are to: 1) clone and biochemically characterize the currently unknown proteins in the gamma-TuRC; 2) elucidate the structural organization of gamma-TuRC; 3)reconstitute functional gamma TuRC in vitro; and 4)determine the detailed functions of the gamma-TuRC.

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