, STRUCTURE OF Y TUBULIN RING COMPLEX

, Y 微管蛋白环复合物的结构

• 批准号: 6444671
• 负责人: Yixian Zheng
• 金额: $ 29.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444671
• 关键词: animal tissue; biomedical resource; genome; growth /development

A study of the shucture and function of a microtubule-nucleating complex from Drosophild embryo extracts was initiated. The y-TuRC (y-Tubulin Ring Complex) appeared as disc-shaped particles in the STEM when the specimen was fi=ze-dried, simila to previous TEM image& The preparation was relatively clean and the complexes were approximately 2.5 MDa. Many additional experiments are planned. It turns out that their preparations routinely have 2kDa peptides (to elute the complex from a column) which has'caused bad background problems. Work is underway to avoid this.

微管成核的结构和功能研究 从果蝇胚胎提取物中提取的复合体被启动。Y-TURC (Y-微管蛋白环复合体)在STEM中以盘状颗粒出现 当样品被烘干时，与之前的透射电子显微镜图像相似& 制剂相对清洁，络合物大致为 2.5丙二醛。还计划进行更多的实验。事实证明， 他们的制剂通常含有2 kDa的多肽(以洗脱复合体 专栏)，这已经造成了糟糕的背景问题。工作是 正在进行中，以避免这种情况。