COMPARE 39K TRANSVERSE RELAXATION IN VITRO SOLUTIONS & IN CYTOPLASM OF E COLI

比较 39K 横向松弛体外解决方案

基本信息

  • 批准号:
    6298205
  • 负责人:
  • 金额:
    $ 0.75万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1999
  • 资助国家:
    美国
  • 起止时间:
    1999-03-01 至 2000-02-29
  • 项目状态:
    已结题

项目摘要

The most abundant cation and osmolyte in the cytoplasm of E. coli, K+, has been used in studies of molecular interactions of DNA, proteins, ribosome and ions via 39K NMR. In our previous experiments, a large transverse relaxation rate of 39K has been observed in dense slurries of E. coli cells. Since the interaction of the K nucleus with the surrounding electric field gradient of the DNA dominants the 39K transverse relaxation in a K-DNA solution with little salt added, it was considered as the explanation for the 39K relaxation measured in vivo. However, the relaxation rate of 39K in DNA solutions, even with a very high concentration (i.e., physiological conditions), is much slower than that in vivo. Similar results were obtained for concentrated K-BSA and KCl solutions. The only in vitro solution that gives rise to the same large magnitude of the transverse relaxation rate as in vivo are ribosome solutions, the highly negative charged ribosome particles that have the most nucleic acids in the cell. Therefore, ribosome particles are considered as the key element that enhances the transverse relaxation of 39K in vivo. The similar characteristic of transverse relaxation was also observed in 23Na-ribosome solutions. These results require further experimental studies to confirm and to explore the molecular nature of the ribosome particle and K+, Na+-ribosome interactions. We will be using the DMX 400 spectrometer with the 10mm BB probe for both 39K and 23Na (a more sensitive nucleus) NMR studies.
大肠杆菌细胞质中最丰富的阳离子和渗透物,

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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M. THOMAS RECORD其他文献

M. THOMAS RECORD的其他文献

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{{ truncateString('M. THOMAS RECORD', 18)}}的其他基金

AS Mechanisms of RNA Polymerase-Promoter and lac Repressor-Operator Interactions
RNA 聚合酶启动子和 lac 抑制子-操纵子相互作用的 AS 机制
  • 批准号:
    9442919
  • 财政年份:
    2016
  • 资助金额:
    $ 0.75万
  • 项目类别:
Mechanisms of RNA Polymerase-Promoter and lac Repressor-Operator Interactions
RNA 聚合酶启动子和 lac 抑制子-操纵子相互作用的机制
  • 批准号:
    9071149
  • 财政年份:
    2016
  • 资助金额:
    $ 0.75万
  • 项目类别:
Roles of RNA Polymerase Downstream Mobile Elements in Transcription Initiati
RNA 聚合酶下游移动元件在转录起始中的作用
  • 批准号:
    8348191
  • 财政年份:
    2012
  • 资助金额:
    $ 0.75万
  • 项目类别:
Roles of RNA Polymerase Downstream Mobile Elements in Transcription Initiati
RNA 聚合酶下游移动元件在转录起始中的作用
  • 批准号:
    8669016
  • 财政年份:
    2012
  • 资助金额:
    $ 0.75万
  • 项目类别:
Roles of RNA Polymerase Downstream Mobile Elements in Transcription Initiati
RNA 聚合酶下游移动元件在转录起始中的作用
  • 批准号:
    8539635
  • 财政年份:
    2012
  • 资助金额:
    $ 0.75万
  • 项目类别:
Solute Effects on Biopolymer Processes
溶质对生物聚合物过程的影响
  • 批准号:
    7928500
  • 财政年份:
    2009
  • 资助金额:
    $ 0.75万
  • 项目类别:
COMPARE 39K TRANSVERSE RELAXATION IN VITRO SOLUTIONS & IN CYTOPLASM OF E COLI
比较 39K 横向松弛体外解决方案
  • 批准号:
    6309208
  • 财政年份:
    2000
  • 资助金额:
    $ 0.75万
  • 项目类别:
COMPARISONS:39K TRANSVERSE RELAXATION IN VITRO SOLUTIONS & E COLI K 12 CYTOPLASM
比较:39K 横向松弛体外解决方案
  • 批准号:
    6120992
  • 财政年份:
    1999
  • 资助金额:
    $ 0.75万
  • 项目类别:
COMPARE 39K TRANSVERSE RELAXATION IN VITRO SOLUTIONS & IN CYTOPLASM OF E COLI
比较 39K 横向松弛体外解决方案
  • 批准号:
    6281614
  • 财政年份:
    1998
  • 资助金额:
    $ 0.75万
  • 项目类别:
39K TRANSVERSE RELAXATION IN VITRO & IN E COLI K 12 CYTOPLASM COMPARISON
体外 39K 横向松弛
  • 批准号:
    6252117
  • 财政年份:
    1997
  • 资助金额:
    $ 0.75万
  • 项目类别:

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