Microbiome Productivity: investigating the photophysiology and productivity of microalgal biomes in coralline algal habitats

微生物组生产力：研究珊瑚藻栖息地微藻生物群落的光生理学和生产力

• 批准号: NE/L012901/1
• 负责人: Rupert Perkins
• 金额: $ 3.19万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FL012901%2F1
• 关键词: Microbiome; Productivity; investigating; photophysiology; productivity

This project utilises the expertise of the UK partners regarding macro and microalgal taxonomy, ecology and physiology, in a novel collaboration with the French partners regarding single cell analysis of samples using the combination of Hyperspectral Imaging and high resolution fluorescence. The subject matter will be the combination of the bioengineer Coralline macroalgae and the epi and endo biotic microalgal biome associated within the fronds of these macroalgae. This is a value added investigation expanding upon a NERC UK Ocean Acidification research Programme studentship project supervised by the UK partners. Coralline macroalgae are calcifying organisms that engineer a 3-dimensional habitat colonised by many endobiotic and epibiotic organisms. A large part of this community is comprised of photosynthetic microalgae. These are important biomes, yet are extremely poorly understood with regard to basic knowledge of community structure, or the functional relevance or interaction of the microalgae and the macroalgae. This project therefore aims to establish a long term collaboration between the UK and French partners, investigating the two photosynthetic components of the biome with regard to community structure, primary productivity and functional photophysiology. Initial pilot data obtained by the PI and the French partners using seagrass (Zostera species) has indicated that paired hyperspectral imaging and high resolution fluorescence imaging produces spectral signatures that can identify the major taxa dominating the epiphytic community growing on the Zostera. Further analysis suggests that this data, paired with high resolution fluorescence may enable analysis of primary productivity, photosynthetic pigments and functional photophysiology (e.g. down regulation, photoacclimation etc.) of individual single cell epiphytes as well as individual segments (intergenicula) of the macroalgal frond. If this proves correct, then the novel pairing of these methods will provide ultra-fine scale data on the photophysiology and productivity of the macroalgae, the microalgae and the interaction between the two. This will greatly expand our understanding of the function of the macro and micro components of these important communities and hence enable a far more informed interpretation of photophysiological data when investigating impacts, such as acidification and light induced stressors, or enhanced carbon dioxide treatments, applied in studies of these important coastal organisms.

该项目利用了英国合作伙伴在宏观和微藻分类学、生态学和生理学方面的专业知识，与法国合作伙伴进行了一项新的合作，利用高光谱成像和高分辨率荧光相结合对样品进行单细胞分析。主题将是生物工程珊瑚大型藻类和这些大型藻类的叶状体内相关的表皮和内生生物微藻生物群系的组合。这是一项增值调查，扩大了由英国合作伙伴监督的NERC英国海洋酸化研究计划学生项目。珊瑚大型藻类是钙化生物，工程师的三维栖息地殖民许多内生和后生生物有机体。这个群落的很大一部分由光合微藻组成。这些都是重要的生物群落，但对群落结构的基本知识，或微藻和大型藻类的功能相关性或相互作用了解甚少。因此，该项目旨在建立英国和法国合作伙伴之间的长期合作，调查生物群落的两个光合组成部分，包括群落结构，初级生产力和功能生物生理学。PI和法国合作伙伴使用海草（Zostera物种）获得的初步试验数据表明，配对的高光谱成像和高分辨率荧光成像产生的光谱特征可以识别在Zostera上生长的附生群落中占主导地位的主要类群。进一步的分析表明，这些数据与高分辨率荧光相结合，可以分析初级生产力、光合色素和功能性光合生理学（例如下调、光适应等）。单个单细胞附生植物以及大型藻类叶的单个节段（膝状体）。如果这被证明是正确的，那么这些方法的新配对将提供关于大型藻类、微藻类的生物生理学和生产力以及两者之间的相互作用的超细尺度数据。这将大大扩大我们对这些重要群落的宏观和微观组成部分的功能的理解，从而在调查影响时，例如酸化和光诱导的压力源，或增强二氧化碳处理，应用于这些重要的沿海生物的研究时，能够对生物生理学数据进行更明智的解释。

项目成果

Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging.

• DOI: 10.1038/srep22343
• 发表时间: 2016-02-29
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Perkins RG;Williamson CJ;Brodie J;Barillé L;Launeau P;Lavaud J;Yallop ML;Jesus B
• 通讯作者: Jesus B