CORE--LABORATORY

计划项目核心(雅奎因、罗得斯)-将有七个设施/功能 提供以下服务： 1)位于圣路易斯的一家用于染色和分析的单一“形态测量”设备 单个三叉神经细胞的长程投射和树突，以及 用于分析三叉神经种群的空间分布格局 神经元，在正常发育期间和在各种实验后 微扰。Jacquin，Arends和Woolsey博士将监督它的使用 计划项目的所有参与者。 2)圣路易斯的一家组织电子显微镜设备 过程中超微结构重组的处理和记录 正常发育和实验操作后。扎姆博士会 监督其使用情况。 3)托莱多的一个单一组织培养设施，用于评估 轴突靶点选择中的传入和靶点相关因素 轴突分枝模式的发展。汉金博士将监督它的 使用。 4)托莱多的一个单独设施，用于住房和准备 实验动物。罗迪斯博士将监督它的使用。 5)及时传递基线数据、实验材料和想法 在不同的实验室之间。 6)由外部咨询委员会和应邀研讨会进行外部审查 扬声器。 7)行政管理。 通过建立这些核心设施和职能，我们： 1)将在一个实验室开发的技术升级提供给所有 组件项目。 2)最大限度地利用个体实验动物增加 得出结论的力量。 3)确保从不同实验室获得的数据 以类似的方式进行比较和分析，以避开共同的 与以下研究结果的相关性相关的陷阱 独立的观察者和不同的分析技术。 4)为试验性和概念性整合提供手段 促进。 5)提供内部和定期安排的定期会议 外部对我们进展的审查。 6)管理各项项目。 所有必要的设备和人员目前都在我们的 计划项目。

Program Project CORE (Jacquin, Rhoades) - Seven facilities/functions will be made available: 1) A single "morphometry" facility in St. Louis for staining and analyzing the long-range projections and dendrites of single trigeminal neurons, and for analyzing spatial distribution patterns of populations of trigeminal neurons, during normal development and after various experimental perturbations. Drs. Jacquin, Arends and Woolsey will oversee its use by all of the Program Project participants. 2) A single electron microscopy facility in St. Louis for tissue processing and documentation of ultrastructural reorganization during normal development and after experimental manipulations. Dr. Zahm will oversee its use. 3) A single tissue culture facility in Toledo for assessing the role of afferent-and target-related factors in axon target selection and the development of axon arborization patterns. Dr. Hankin will oversee its use. 4) A single facility in Toledo for the housing and preparation of experimental animals. Dr Rhoades will oversee its use. 5) Timely transmission of baseline data, experimental materials and ideas between the different laboratories. 6) External review by an External Advisory Committee and invited seminar speakers. 7) Administration. By instituting these CORE facilities and functions we: 1) Make technical upgrades developed in one laboratory available to all of the component projects. 2) Make optimum use of individual experimental animals to increase the power of conclusions drawn. 3) Ensure that the data obtained from the different laboratoaries are comparable and analysed in a like manner so as to circumvent common pitfalls associated with the correlation of findings obtained by independent observers and different analytic techniques. 4) Provide means for experimental and conceptual integration and facilitation. 5) Provide frequent and regularly scheduled meetings for internal and external reviews of our progress. 6) Administer the various projects. All requisite equipment and personnel are currently funcitoning within our Program Project.