CORE-- IMAGE ANALYSIS AND MORPHOMETRY

核心——图像分析与形态测量

• 批准号: 6868899
• 负责人: MARK F JACQUIN
• 金额: $ 3.77万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868899
• 关键词: bioimaging /biomedical imaging; biomedical facility; image processing; microscopy; morphometry

The Image Analysis and Quantitative Morphometry Core provides Program Project Investigators a wide variety of options for the quantitative analysis from histological preparations. The Core Resource has: a Zeiss LSM (laser scanning microscope) confocal microscope; several research microscopes interfaced for photography, digital image capture and computer driven stage control; image digitization from microscopes photographs and drawings; CD writing for archival storage; up to date generate , special purpose and locally modified commercial and public domain software; and network connections to all investigators The resources evaluates: regional areas and volumes; projection (whisker like) patterns; neuron number/phenotype; relative message and protein density; cell death; and process length, branching and target size. Materials and images are evaluated directly by expert Core morphologists (blinded to experimental conditions when necessary) and analyzed by appropriate statistics in consultation with the Core statistician. The Core contributes intellectually in preparation of materials for presentation and publication and makes illustrations for manuscripts and 35 mm sides. The Core plans to modernize some of its special purpose hardware and software. The Core streamlines data collection by writing macros. The Core will facilitate Program Project communication via its web page. This Core contributes to the general theme of this Program Project which is directed to understanding mechanisms of developmental plasticity as a basis for rational therapy of conditions affecting the nervous system, especially as they relate to orofacial sensation.

图像分析和定量形态测量核心为计划项目调查人员提供了从组织学准备进行定量分析的广泛选择。核心资源有：蔡司LSM(激光扫描显微镜)共焦显微镜；用于摄影、数字图像捕获和计算机驱动舞台控制的几台研究显微镜；从显微镜照片和图纸进行图像数字化；用于档案存储的CD写入；最新生成、特殊用途和本地修改的商业和公共领域软件；以及与所有研究人员的网络连接。该资源评估：区域面积和体积；投影(晶须状)图案；神经元数量/表型；相对信息和蛋白质密度；细胞死亡；以及突起长度、分支和目标大小。材料和图像由专业的核心形态专家直接评估(必要时对实验条件视而不见)，并在咨询核心统计师的情况下通过适当的统计进行分析。该中心在准备演示和出版材料方面做出了智力贡献，并为手稿和35毫米的侧面制作插图。Core计划对其一些特殊用途的硬件和软件进行现代化改造。Core通过编写宏来简化数据收集。核心将通过其网页促进计划项目的沟通。这一核心有助于该计划项目的总主题，旨在了解发育可塑性的机制，作为合理治疗影响神经系统的条件的基础，特别是当它们与口面部感觉有关时。