CORE-- IMAGE ANALYSIS AND MORPHOMETRY

核心——图像分析与形态测量

• 批准号: 6868899
• 负责人: MARK F JACQUIN
• 金额: $ 3.77万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868899
• 关键词: bioimaging /biomedical imaging; biomedical facility; image processing; microscopy; morphometry

The Image Analysis and Quantitative Morphometry Core provides Program Project Investigators a wide variety of options for the quantitative analysis from histological preparations. The Core Resource has: a Zeiss LSM (laser scanning microscope) confocal microscope; several research microscopes interfaced for photography, digital image capture and computer driven stage control; image digitization from microscopes photographs and drawings; CD writing for archival storage; up to date generate , special purpose and locally modified commercial and public domain software; and network connections to all investigators The resources evaluates: regional areas and volumes; projection (whisker like) patterns; neuron number/phenotype; relative message and protein density; cell death; and process length, branching and target size. Materials and images are evaluated directly by expert Core morphologists (blinded to experimental conditions when necessary) and analyzed by appropriate statistics in consultation with the Core statistician. The Core contributes intellectually in preparation of materials for presentation and publication and makes illustrations for manuscripts and 35 mm sides. The Core plans to modernize some of its special purpose hardware and software. The Core streamlines data collection by writing macros. The Core will facilitate Program Project communication via its web page. This Core contributes to the general theme of this Program Project which is directed to understanding mechanisms of developmental plasticity as a basis for rational therapy of conditions affecting the nervous system, especially as they relate to orofacial sensation.

图像分析和定量形态测定核心为项目研究人员提供了多种用于组织学制剂定量分析的选项。核心资源有：蔡司LSM（激光扫描显微镜）共焦显微镜；多个研究显微镜，用于摄影、数字图像捕捉和计算机驱动的载物台控制；显微镜照片和图纸的图像数字化；用于存档存储的 CD 刻录；最新生成的、特殊用途的和本地修改的商业和公共领域软件；以及与所有调查人员的网络连接 资源评估：区域面积和数量；投影（晶须状）图案；神经元数量/表型；相对信息和蛋白质密度；细胞死亡；以及过程长度、分支和目标大小。材料和图像由专家核心形态学家直接评估（必要时对实验条件不知情），并与核心统计学家协商通过适当的统计数据进行分析。核心在准备演示和出版材料方面做出智力贡献，并为手稿和 35 毫米边制作插图。 Core 计划对其一些特殊用途的硬件和软件进行现代化改造。核心通过编写宏来简化数据收集。核心将通过其网页促进计划项目的沟通。该核心有助于该计划项目的总体主题，该主题旨在理解发育可塑性的机制，作为影响神经系统的疾病（尤其是与口面部感觉相关的疾病）合理治疗的基础。