CORE-- MORPHOLOGY

核心——形态学

• 批准号: 6868897
• 负责人: MARK F JACQUIN
• 金额: $ 3.07万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868897
• 关键词: biomedical facility; electron microscopy; histology; morphology; stainings

This CORE will continue to have as its general aim to facilitate and ensure the accomplishment of the individual projects' Specific Aims. In addition, the Core will provide centralized facilities at Washington University for staining neurons, ultrastructural analyses, and distribution of experimental animals that are used in more than one project.. As such, the CORE will: 1) make technical expertise available to all of the component projects, 2) make optimum use of individual experimental animals, 3) ensure that the data obtained from the different laboratories are comparable and analyzed in a like manner so as to circumvent common pitfalls associated with the correlation of findings obtained by independent observers and different analytic techniques, 4) administer the various projects, and 5) provide means for experimental and conceptual integration and external review. To accomplish these Specific Aims, this CORE will carry out 5 different functions. These are: 1. A single facility in St. Louis for staining the long-range projections and dendrites of somatosensory neurons in various experimental preparations- The CORE will operate a facility in St. Louis that will be used by Project #s1, 2, 4, and 5 to stain the processes of single neurons at various levels of somatosensory neuraxis. 2. A single electron microscopy facility in St. Louis for tissue processing and documentation of ultrastructure- Two projects in St. Louis (#s 1 and 4) require electron microscopy. We proposed to continue with Mr. Joseph DeMaro as the CORE EM technician and associated in place equipment to provide an avenue for study of central ultrastructural development under conditions of neurotrophin augmentation and stem cell transplantation. 3. Timely transmission of data and ideas between the different laboratories-The projects comprising this proposal are closely interrelated technically and conceptually. Scheduled bimonthly meetings will ensure that necessary information, materials and critical reviews are transmitted between laboratories on a regular basis. 4. Continual external review of progress and results-This aim will continue to be met by establishing new and appropriate internal and external advisory groups that will meet annually to evaluate our work. Experts in the field of neuronal development will also be invited to give presentations and visit less formally with project investigators. 5. Administration All requisite and personnel are currently functioning without our Program Project.

这一核心将继续以促进和确保实现个别项目的具体目标为总目标。此外，核心将在华盛顿大学提供集中式设施，用于神经元染色、超微结构分析和实验动物的分布，这些实验动物用于不止一个项目。因此，核心将：1)向所有组成部分项目提供技术专长；2)最大限度地利用单个实验动物；3)确保从不同实验室获得的数据具有可比性，并以同样的方式进行分析，以避免与独立观察员获得的结果和不同分析技术相关联的常见陷阱；4)管理各种项目；5)提供实验和概念整合以及外部审查的手段。为了实现这些具体目标，这个核心将执行5个不同的功能。这些是：1.圣路易斯的一个单一设施，用于在各种实验准备中对体感神经元的远程投射和树突进行染色--核心将在圣路易斯运营一个设施，项目#S1、2、4和5将使用该设施来染色不同水平的躯体感觉神经轴上的单个神经元的突起。2.圣路易斯的一个用于组织处理和超微结构记录的单一电子显微镜设施--圣路易斯的两个项目(#S 1和#4)需要电子显微镜。我们建议约瑟夫·德马罗先生继续担任EM核心技术人员和相关设备，为研究神经营养素增强和干细胞移植条件下的中枢超微结构发育提供途径。3.在不同的实验室之间及时传递数据和想法--组成这项提案的项目在技术和概念上密切相关。预定的双月会议将确保在实验室之间定期传递必要的信息、材料和关键审查。4.对进展和成果进行持续的外部审查--将继续通过设立新的适当的内部和外部咨询小组来实现这一目标，这些小组将每年举行会议，以评价我们的工作。神经元发育领域的专家也将被邀请发表演讲，并与项目调查人员进行不那么正式的访问。5.管理目前在没有我们的计划项目的情况下，所有必需的人员都在工作。