CORE-- MORPHOLOGY

核心——形态学

• 批准号: 6868897
• 负责人: MARK F JACQUIN
• 金额: $ 3.07万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868897
• 关键词: biomedical facility; electron microscopy; histology; morphology; stainings

This CORE will continue to have as its general aim to facilitate and ensure the accomplishment of the individual projects' Specific Aims. In addition, the Core will provide centralized facilities at Washington University for staining neurons, ultrastructural analyses, and distribution of experimental animals that are used in more than one project.. As such, the CORE will: 1) make technical expertise available to all of the component projects, 2) make optimum use of individual experimental animals, 3) ensure that the data obtained from the different laboratories are comparable and analyzed in a like manner so as to circumvent common pitfalls associated with the correlation of findings obtained by independent observers and different analytic techniques, 4) administer the various projects, and 5) provide means for experimental and conceptual integration and external review. To accomplish these Specific Aims, this CORE will carry out 5 different functions. These are: 1. A single facility in St. Louis for staining the long-range projections and dendrites of somatosensory neurons in various experimental preparations- The CORE will operate a facility in St. Louis that will be used by Project #s1, 2, 4, and 5 to stain the processes of single neurons at various levels of somatosensory neuraxis. 2. A single electron microscopy facility in St. Louis for tissue processing and documentation of ultrastructure- Two projects in St. Louis (#s 1 and 4) require electron microscopy. We proposed to continue with Mr. Joseph DeMaro as the CORE EM technician and associated in place equipment to provide an avenue for study of central ultrastructural development under conditions of neurotrophin augmentation and stem cell transplantation. 3. Timely transmission of data and ideas between the different laboratories-The projects comprising this proposal are closely interrelated technically and conceptually. Scheduled bimonthly meetings will ensure that necessary information, materials and critical reviews are transmitted between laboratories on a regular basis. 4. Continual external review of progress and results-This aim will continue to be met by establishing new and appropriate internal and external advisory groups that will meet annually to evaluate our work. Experts in the field of neuronal development will also be invited to give presentations and visit less formally with project investigators. 5. Administration All requisite and personnel are currently functioning without our Program Project.

该核心方案的总体目标将继续是促进和确保实现各个项目的具体目标。此外，核心将在华盛顿大学提供集中设施，用于神经元染色、超微结构分析和分配用于多个项目的实验动物。因此，核心小组将：1）为所有组成项目提供技术专业知识，2）最佳利用单个实验动物，3）确保从不同实验室获得的数据具有可比性，并以类似的方式进行分析，以避免与独立观察员和不同分析技术获得的结果相关的常见陷阱，4）管理各种项目，5）提供实验和概念整合以及外部审查的手段。为了实现这些具体目标，该CORE将执行5个不同的功能。这些是：1.圣路易斯的一个单一设施，用于染色各种实验制剂中的体感神经元的长程投射和树突-CORE将在圣路易斯运营一个设施，该设施将用于项目#s1、2、4和5，用于染色体感神经轴不同水平的单个神经元的过程。2.圣路易斯的一个电子显微镜设施，用于组织处理和超微结构记录-圣路易斯的两个项目（#s 1和4）需要电子显微镜。我们建议继续由Joseph DeMaro先生担任核心EM技术员，并配备相关设备，为神经营养因子增强和干细胞移植条件下的中枢超微结构发育研究提供途径。3.在不同实验室之间及时传输数据和想法--本提案所包含的项目在技术和概念上密切相关。预定的双月会议将确保实验室之间定期传递必要的信息、材料和重要评论。4.对进展和结果进行持续的外部审查-这一目标将继续通过建立新的和适当的内部和外部咨询小组来实现，这些小组将每年开会评估我们的工作。还将邀请神经元发育领域的专家进行演讲，并与项目研究人员进行非正式访问。5.管理所有必需品和人员目前没有我们的计划项目运作。