BIOLOGICAL MASS SPECTROMETRY SYSTEMS

最新的基质辅助激光解吸飞行时间 (MALDI-TOF)和台式电喷雾电离(ESI)质量 将购买光谱仪，以进一步研究 设施、博士后助理和研究生 化学、生物化学、细胞和结构系 生物学。该系统将用于生物学研究。 高分子量的大分子。有一系列的 不能用当前质量进行的重要测量 分光计，因此这项购买成为可能的广泛范围 项目的数量。新乐器将被安置在 化学科学在其质谱学实验室。 系统的主要用户将是M.Gillette来研究角色 大脑中的多肽~S生物钟，J.A.卡森伦伯根 分析配体诱导的构象变化和磷酸化 在雌激素受体方面，D.Kranz研究了T细胞的调控 多肽/蛋白质激动剂和拮抗剂的活性，J.S.摩尔 表征结构控制的大分子，K.L.莱因哈特 先进的高分辨率生物质谱学，K.S.Suslick to 研究血红素蛋白和金属卟啉，以及J.V.Sweedler到 研究多肽在细胞内的分布 单个神经元的突起。斯莱格教授，虽然只是个未成年人 建议工具的使用者，是学校的主任 伊利诺伊大学的化学科学，因此是 这项提案的首席调查员。

State-of-the-art matrix-assisted laser desorption time-of-flight (MALDI-TOF) and bench-top electrospray ionization (ESI) mass spectrometers will be purchased to further the research of the facility, postdoctoral associates, and graduate students in the Departments of Chemistry, Biochemistry, and Cell and Structural Biology. The systems will be used for the study of biological macromolecules of high molecular weight. There are a series of important measurements that cannot be undertaken with current mass spectrometers, and so this purchase becomes enabling for a wide range of projects. The new instruments will be housed in the School of Chemical Sciences in its Mass Spectrometry laboratory. The major users of the systems will be M. Gillette to study the roles of peptides in the brain~s biological clocks, J.A. Katzenellenbogen to analyze ligand-induced conformational changes and phosphorylation in estrogen receptors, D. Kranz to investigate the control of T-cell activity with peptide/protein agonists and antagonists, J.S. Moore to characterize structure-controlled macromolecules, K.L. Rinehart to advance high resolution biological mass spectrometry, K.S. Suslick to study heme proteins and metalloporphyins, and J.V. Sweedler to investigate the distribution of peptides throughout the cellular processes of individual neurons. Prof. Sligar, although only a minor user of proposed instruments, is the director of the School of Chemical Sciences at the University of Illinois and therefore is the Principal Investigator of this proposal.