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PROBING HEME ENVIRONMENT IN RECOMBINANT HUMAN HEMOGLOBIN PRODUCTS

PROBING HEME ENVIRONMENT IN RECOMBINANT HUMAN HEMOGLOBIN PRODUCTS
探索重组人血红蛋白产品中的血红素环境
批准号：
6120649
负责人：
STEPHEN G. SLIGAR
金额：
$0.27万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-04-15 至 1999-11-30
来源：
https://reporter.nih.gov/project-details/6120649
关键词：
biological products biomedical resource proteins

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英文摘要
We use EPR spectroscopy to probe the environment of the heme moity in recombinant adult hemoglobin produced in E. coli. UV and visible spectroscopy suggest that some portion of the protein subunits may possess a sulfur-derivated heme prosthetic group described elsewhere as sulf-hemoglobin. This small (5%) contaminating amount of sulf-heme may explain the reduced cooperativity of hemoglobin produced by bacterial expression. EPR analysis shows significant differences in the low spin hydroxy bound species in the g=2 area of the spectra between native and recombinant.
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