CORE--TISSUE CULTURE

细胞培养核心将为 通过帮助调查人员执行和解释结果来实现SCOR项目 他们的造血细胞培养实验。 将在以下方面提供科学和技术支持 方式： A)核心单位的项目负责人将提供一个集中的 该SCOR的科学家所处的科学和技术环境 将能够讨论和设计他们的实验的细胞生物学 计划。她还将提供细胞培养方面的实验培训。 给那些认为实验应该是 在他们自己的实验室里进行。 B)核心的技术支持将是： 从造血组织(脐带血、成人血或 骨髓、胎肝和胎儿血液)来自正常人或 具有特定血型的个体(例如，Kell-Null 使用免疫亲和层析(Cellpro设备)。 不能加工的此类样品的超低温保存和储存 立即并维护样本库的记录。 祖细胞测定及液体培养扩增已确定细胞 培养条件下的谱系(红系、髓系和肥大细胞) 这将仅允许扩展一个定义良好的差异化 血统。核心还将建立和维持基质细胞系 取自正常骨髓。 初级试验用化学试剂和细胞试剂的制备 造血培养。

The Cell Culture Core will provide scientific and technical support to the SCOR project by helping investigators implement and interpret the results of their hematopoietic cell culture experiments. The scientific and technical support will be provided in the following ways: a) The Project Leader of the Core unit will provide a centralized scientific and technical environment in which the scientists of this SCOR would be able to discuss and design the cell biology of their experimental plans. She will also provide experimental training in cell culture procedures to investigators who feel that the experiments should be carried out in their own laboratories. b) The technical support of the Core will be: CD34+ isolation from hematopoietic tissues (cord blood, adult blood or marrow, fetal liver and fetal blood) from normal individuals or from individuals with a particular blood phenotype (for example, Kell-null using immunoaffinity chromatography (Cellpro devices). Cryopreservation and storage of such samples which cannot be processed immediately and maintaining records of the sample bank. Progenitor cell assay and liquid culture expansion of defined cell lineages (erythroid, myeloid and mast cells) under culture conditions which would allow the expansion of only one well defined differentiation lineage. The core will also establish and maintain stromal cell lines from normal bone marrow. Preparation of tested chemical and cellular reagents for primary hematopoietic cultures.