CORE--TISSUE CULTURE

核心——组织培养

• 批准号: 6302334
• 负责人: Anna Rita F Migliaccio
• 金额: $ 23.65万
• 依托单位: NEW YORK BLOOD CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302334
• 关键词: biomedical facility; cryopreservation; hematopoietic tissue; human fetus tissue; human tissue; tissue /cell culture; tissue /cell preparation

The Cell Culture Core will provide scientific and technical support to the SCOR project by helping investigators implement and interpret the results of their hematopoietic cell culture experiments. The scientific and technical support will be provided in the following ways: a) The Project Leader of the Core unit will provide a centralized scientific and technical environment in which the scientists of this SCOR would be able to discuss and design the cell biology of their experimental plans. She will also provide experimental training in cell culture procedures to investigators who feel that the experiments should be carried out in their own laboratories. b) The technical support of the Core will be: CD34+ isolation from hematopoietic tissues (cord blood, adult blood or marrow, fetal liver and fetal blood) from normal individuals or from individuals with a particular blood phenotype (for example, Kell-null using immunoaffinity chromatography (Cellpro devices). Cryopreservation and storage of such samples which cannot be processed immediately and maintaining records of the sample bank. Progenitor cell assay and liquid culture expansion of defined cell lineages (erythroid, myeloid and mast cells) under culture conditions which would allow the expansion of only one well defined differentiation lineage. The core will also establish and maintain stromal cell lines from normal bone marrow. Preparation of tested chemical and cellular reagents for primary hematopoietic cultures.

细胞培养中心将为 SCOR项目，帮助研究人员实施和解释结果 他们的造血细胞培养实验。 将在以下方面提供科学和技术支助 方式： a）核心单位的项目负责人将提供一个集中的 科学和技术环境中，这个SCOR的科学家 将能够讨论和设计他们实验的细胞生物学， 布局 她还将提供细胞培养方面的实验培训 研究人员认为，实验应该 在他们自己的实验室里进行。 B）核心的技术支持将是： 从造血组织（脐带血、成人血或 骨髓、胎肝和胎血）或来自 具有特定血液表型的个体（例如，Kell-null 使用免疫亲和层析（Cellpro装置）。 冷冻保存和储存无法处理的样本 并保存样品库的记录。 祖细胞测定和确定细胞的液体培养扩增 培养条件下的谱系（红细胞、髓细胞和肥大细胞） 这将允许仅一个明确定义的分化的扩展 脉 核心还将建立和维持基质细胞系 从正常的骨髓中提取 制备供试化学试剂和细胞试剂， 造血培养物