IMAGING OXYGEN METABOLISM IN BRAIN

描述（改编自申请人摘要）：缺血性卒中是一种 常见的毁灭性疾病 该项目的长期目标是 开发一种工具来研究中风的病理生理学，特别是 缺血半暗带 在缺血中心血流量<20%的正常 几乎没有新陈代谢。 周边地区也减少了 血流量（正常的20%至50%），但只有轻微的代谢不足。 这个区域被称为“缺血半暗带”。 事实表明， 可以通过早期治疗挽救该区域。 这些领域也涉及 在活性蛋白质合成中起重要作用，而活性蛋白质合成被认为赋予缺血耐受性。 申请人假设，在体内磁共振成像（MRI）， 耗氧量可以区分半暗区和梗死区 在局灶性缺血的情况下。 为了验证这一假设， 申请人提出开发新的MR方法来对脑氧进行成像 新陈代谢和血液流动。 这些方法是基于间接检测 17-0核使用1H MRI，这已经被证明是一个 敏感技术 申请人将在正常大鼠中评价该方法 并与现有的测量脑氧含量的方法进行对比， 新陈代谢. 然后，他将使用这种方法来研究一个可逆的模型， 涉及大脑中动脉阻塞的中风。 现象 将通过将MR数据与 免疫组织化学分析用于蛋白质表达。 他希望通过这种方式， 能够将MR图像与蛋白质合成相关联。 该项目将 大大提高了我们对中风病理生理学的理解， 可能提供一种工具， 治疗

DESCRIPTION (Adapted from applicant's abstract): Ischemic stroke is a common and devastating disorder. The long-term goal of this project is to develop a tool to study the pathophysiology of stroke and in particular the ischemic penumbra. In the ischemic core blood flow is <20 percent of normal and metabolism is almost absent. The peripheral regions also have reduced blood flow (20 to 50 percent of normal) but are only slightly hypometabolic. This region is called the "ischemic penumbra". It has been shown that this region can be salvaged with early treatment. These areas are also involved in active protein synthesis which is thought to endow ischemic tolerance. The applicant hypothesizes that in vivo magnetic resonance imaging (MRI) of oxygen consumption can distinguish penumbral regions from infarcted regions in the setting of focal ischemia. In order to test this hypothesis the applicant proposes to develop novel MR methods to image cerebral oxygen metabolism and blood flow. The methods are based on indirect detection of the 17-0 nucleus using 1H MRI, which has already been demonstrated to be a sensitive technique. The applicant will evaluate this method in normal rats and validate it against existing methods for measuring cerebral oxygen metabolism. He will then use this method to study a reversible model of stroke involving occlusion of middle cerebral artery. The phenomenon of ischemic tolerance will be investigated by correlating MR data with immunohistochemical assays for protein expression. In this way, he hopes to be able to correlate MR images with protein synthesis. This project will greatly enhance our understanding of the pathophysiology of stroke and possibly provide a tool with which to noninvasively assess genetic therapies.