CORE--ANALYTICAL

描述：开发的分析核心设施提供 强大的中央分析核心，配备各种仪器， 定量分析，供项目中的所有研究者使用 项目核心提供设施和培训， 根据需要，进行计算机图像分析， 放射自显影数据，如凝胶、放射性配体结合和原位 免疫标记的杂交、定量形态测定分析和 常规染色的组织切片和荧光比率成像。 三个计算机化的图像分析系统可用于密度测定 宏观放射自显影样品分析，Zeiss Kontron KS 400 Image 分析系统，主要用于定量形态测量 明场和荧光显微镜图像的分析，以及蔡司 Attofluar钙成像系统。富士BAS 2500荧光成像扫描仪 将被添加到MCID/M4系统，以提高定量 核心的能力;这台扫描仪将被所有的 拟议项目。在核心A中还提供了两个暗室。博士 核心负责人Wilson将协助研究者进行设计， 实现计算机图像分析协议并设置策略， 共享使用设施。分析血液中的铅含量， 通过核心A为所有研究者提供组织样本， 微量金属实验室有三种分析方法可用;原子 吸收光谱学将是该计划使用的主要方法 项目布雷斯勒博士，微量金属实验室副主任， 将监督铅管理方案， 血液/组织铅分析。因此，核心A将提供重要的服务， 铅含量测定，并提供最先进的图像 分析设备，将用于各种定量 个别项目的评估。

DESCRIPTION: The analytical core facilities have been developed to provide a strong central analytical core, with a variety of instrumentation for quantitative analysis, for use for all investigators in the program project. The core provides facilities and training that is used by each of the projects as required, for computerized image analysis of autoradiographic data such as gels, radioligand binding and in the situ hybridization, quantitative morphometric analysis of immunolabeled and conventionally stained tissue sections, and fluorescence ratio imaging. Three computerized image analysis systems are available for densitometric analysis for macro-autoradiographic specimens, a Zeiss Kontron KS400 Image Analysis System, which is used primarily for quantitative morphometric analysis of brightfield and fluorescence microscope images, and a Zeiss Attofluar Calcium Imaging System. A Fuji BAS 2500 Phosphor Imaging Scanner will be added to the MCID/M4 system to enhance the quantitative capabilities of the core; this scanner will be utilized by all of the proposed projects. Two darkrooms are also provided in the core A. Dr. Wilson, core leader, will assist investigator with the design and implementation of computer image analysis protocols and set policies for shared use of the facilities. Analysis of lead levels in the blood and tissue samples is provided through core A for all investigators, in the trace metals laboratory. Three methods of analysis are available; atomic absorption spectroscopy will be the primary method used by the program projects. Dr. Bressler, Associate Director of the trace metals laboratory, will provide oversight of protocols for lead administration and blood/tissue lead analysis. Core A will thus provide an important service, determination of lead levels, and provide access to state-of-the-art image analysis equipment that will be used for a wide variety of quantitative assessments by individual projects.