CORE--ANALYTICAL

描述：开发了分析核心设施，以提供 强大的中央分析核心，具有多种仪器，可用于 定量分析，供项目中的所有研究人员使用 项目。核心提供设施和培训，供每个 按要求进行计算机图像分析的项目 放射自显影数据，如凝胶、放射性配基结合和原位 免疫标记和定量形态计量学的杂交 常规染色组织切片和荧光比值成像。 有三种计算机图像分析系统可用于密度测量 蔡司Kontron KS400图像宏观放射自显影样品的分析 分析系统，主要用于定量形态测量 明场和荧光显微镜图像的分析，以及蔡司 Attoflar钙质成像系统。富士BAS 2500荧光粉成像仪 将被添加到MCID/M4系统，以增强量化 核心的能力；这个扫描仪将被所有 建议的项目。在核心还提供了两个暗室。 威尔逊，核心领导，将协助调查人员设计和 实施计算机图像分析协议并为其制定政策 共享使用这些设施。血铅和血铅含量的分析 组织样本通过核心A提供给所有研究人员，在 痕量金属实验室。有三种分析方法可用；原子分析 吸收光谱学将是该计划使用的主要方法 项目。痕量金属实验室副主任布雷斯勒博士， 将监督领导管理的协议和 血铅/组织铅分析。因此，核心A将提供重要的服务， 铅水平的测定，并提供访问最先进的图像 将用于各种定量分析的分析设备 按个别项目进行评估。

DESCRIPTION: The analytical core facilities have been developed to provide a strong central analytical core, with a variety of instrumentation for quantitative analysis, for use for all investigators in the program project. The core provides facilities and training that is used by each of the projects as required, for computerized image analysis of autoradiographic data such as gels, radioligand binding and in the situ hybridization, quantitative morphometric analysis of immunolabeled and conventionally stained tissue sections, and fluorescence ratio imaging. Three computerized image analysis systems are available for densitometric analysis for macro-autoradiographic specimens, a Zeiss Kontron KS400 Image Analysis System, which is used primarily for quantitative morphometric analysis of brightfield and fluorescence microscope images, and a Zeiss Attofluar Calcium Imaging System. A Fuji BAS 2500 Phosphor Imaging Scanner will be added to the MCID/M4 system to enhance the quantitative capabilities of the core; this scanner will be utilized by all of the proposed projects. Two darkrooms are also provided in the core A. Dr. Wilson, core leader, will assist investigator with the design and implementation of computer image analysis protocols and set policies for shared use of the facilities. Analysis of lead levels in the blood and tissue samples is provided through core A for all investigators, in the trace metals laboratory. Three methods of analysis are available; atomic absorption spectroscopy will be the primary method used by the program projects. Dr. Bressler, Associate Director of the trace metals laboratory, will provide oversight of protocols for lead administration and blood/tissue lead analysis. Core A will thus provide an important service, determination of lead levels, and provide access to state-of-the-art image analysis equipment that will be used for a wide variety of quantitative assessments by individual projects.