ORGANIZATION OF MITOCHONDRIA DURING DVMT OF HAMSTER PREIMPLANTATION EMBRYO

我们正在研究线粒体的组织， 仓鼠植入前胚胎的发育。 的发展 包括仓鼠在内的许多物种的植入前胚胎， 在体外不理想。 最近培养能力的提高 胚胎发生的主要原因是为了理解能量 基板要求。 线粒体的活性是 部分植入前胚胎代谢，可能会受到不利影响 不适当的培养条件。 多光子显微镜 用Rh 123、NAO和1~fitotracker荧光探针， 研究（1）活性线粒体的分布与所有 线粒体在整个胚胎发生的特定点， 仓鼠和（2）培养条件对定位的影响 仓鼠胚胎线粒体的活性。 使用 多光子显微镜将是这项研究的关键，因为我们需要 非常低的光毒性水平，以维持 胚胎。 收集的信息将有助于 了解着床前代谢的调节 仓鼠胚胎，并最终导致改善的能力， 胚胎体外发育

We are studying the organization of mitochondria during the development of the hamster preimplantation embryo. The development of preimplantation embryos from many species including the hamster is suboptimal in vitro. Recent improvement in the ability to culture embryos has occunrd largely because of efforts to understand energy substrate requirements. Nfitochondrial activity which is an integral part of preimplantation embryo metabolism, may be adversely affected by inappropriate culture conditions. Multiphoton microscopy with the fluorescent probes Rh 123, NAO and 1~fitotracker win be used to investigate (1) the distribution of active mitochondria versus all mitochondria at specific points throughout embryogenesis in the hamster and (2) the effects of culture conditions on the localization and activity of mitochondria in the hamster embryos. The use of multiphoton microscopy will be crucial for this study as we require very low levels of phototoxicity in order to maintain the viability of the the embryos. The information gathered will help in the understanding of the regulation of metabolism of the preimplantation hamster embryo and ultimately result in improved ability to support embryo development in vitro.