IMMUNOLOGY & PATHOGENESIS OF INFLUENZA VIRUSES W/ HIGH RESOLUTION CRYO SEM

免疫学

• 批准号: 6117273
• 负责人: VIRGINIA S HINSHAW
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117273
• 关键词: bioimaging /biomedical imaging; biomedical resource; infection; microscopy; virus

Hemagglutinin and neuraminidase are surface attachment proteins of the influenza virus. There are several areas related to these surface proteins which still need to be explored: 1) The distribution of the hemagglutinin and neuraminidase on the viral surface are not yet clear due to the variety of their shapes. 2) The conformational change of hernagglutinin at low pH, which has been indirectly illustrated in other studies. 3) Why the influenza viruses from different species vary in their virulence and their requirements for cleavage of the hemagglutinin molecule. 4) Finally, the interaction of the hemagglutinin with antibody. The examination of influenza virus with high resolution cryo-SEM in combination with backscattered electron (BSE) imaging would be extremely informative from several different perspectives that are related both to antigenic recognition and pathogenesis.

血凝素和神经氨酸酶是一种表面附着蛋白 流感病毒。有几个区域与这些曲面相关 仍需探索的蛋白质：1)蛋白质的分布 病毒表面的血凝素和神经氨酸酶尚不清楚 因为它们的形状多种多样。2)构象变化 在低pH值下的血凝素，这已经在 其他研究。3)为什么不同物种的流感病毒 它们的毒力和它们对裂解的要求不同 血凝素分子。4)最后， 血凝素与抗体。流行性感冒病毒的检测 结合背散射电子的高分辨率低温扫描电子显微镜 (BSE)成像将从几个不同的角度提供极其丰富的信息 与抗原识别和抗原识别有关的观点 发病机制。