IMMUNOLOGY & PATHOGENESIS OF INFLUENZA VIRUSES W/ HIGH RESOLUTION CRYO SEM

免疫学

• 批准号: 6117273
• 负责人: VIRGINIA S HINSHAW
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117273
• 关键词: bioimaging /biomedical imaging; biomedical resource; infection; microscopy; virus

Hemagglutinin and neuraminidase are surface attachment proteins of the influenza virus. There are several areas related to these surface proteins which still need to be explored: 1) The distribution of the hemagglutinin and neuraminidase on the viral surface are not yet clear due to the variety of their shapes. 2) The conformational change of hernagglutinin at low pH, which has been indirectly illustrated in other studies. 3) Why the influenza viruses from different species vary in their virulence and their requirements for cleavage of the hemagglutinin molecule. 4) Finally, the interaction of the hemagglutinin with antibody. The examination of influenza virus with high resolution cryo-SEM in combination with backscattered electron (BSE) imaging would be extremely informative from several different perspectives that are related both to antigenic recognition and pathogenesis.

血凝素和神经氨酸酶是表面附着蛋白 流感病毒。 有几个领域与这些表面相关 仍需探索的蛋白质：1） 病毒表面的血凝素和神经氨酸酶尚不清楚 由于它们的形状多种多样。 2）构象变化 低 pH 条件下的凝集素，已间接说明 其他研究。 3）为什么流感病毒来自不同物种 它们的毒力和裂解要求各不相同 血凝素分子。 4）最后，交互 血凝素与抗体。 流感病毒检查 高分辨率冷冻扫描电镜与背散射电子相结合 (BSE) 成像将通过几种不同的方式提供非常丰富的信息 与抗原识别相关的观点 发病。