EFFECT OF FAT AND FIBER ON COLONIC INTESTINAL EPITHELIAL CELLS

这项研究考察了不同水平的膳食脂肪和 纤维对结肠上皮细胞增殖和死亡的影响。 给予受试者良好控制的饮食（体重维持、代谢）， 具有脂肪/纤维组合（高、正常或低脂肪和高、正常或低脂肪）， 低纤维）作为自变量。 入院时，基线后 内窥镜活检（通过柔性乙状结肠镜），将受试者放置在 低脂肪、高纤维饮食4周。结肠上皮的评估， 丁酸钠和Ki-ras癌基因突变， 内窥镜活检血液和粪便样本 研究意义：结肠 癌症是一种常见的人类恶性肿瘤。 膳食脂肪和纤维 会影响结直肠癌的发病率 消费人口 高脂肪和低纤维的饮食总体上有更高的发病率， 结直肠癌，与消耗较少脂肪和更多脂肪的人群相比， 光纤 目前还不完全清楚膳食脂肪和纤维是否 结肠癌发生的独立变量。 钠 丁酸，一种短链脂肪酸，在结肠中大量产生， 大量的膳食纤维被结肠细菌发酵。 钠 丁酸盐在体外可减少细胞增殖并诱导细胞凋亡。 培养的结肠癌细胞 未来计划：一旦足够的主题 研究人群，实验室和统计分析， 完成后，我们将能够确定膳食脂肪的影响， 纤维对结肠粘膜的几个参数，这可能是重要的 结肠癌风险的指标。 其他研究将重点关注 脂肪和纤维的水平，产生结肠癌的风险最高， 最终分离出这些食物中的物质 结肠癌的易感性

This research examines the effect of different levels of dietary fat and fiber on the proliferation and death of colonic epithelial cells. Subjects are given a well-controlled diet (weight maintaining, metabolic), with fat/fiber combination (high, normal or low fat and high, normal or low fiber) as the independent variable. Upon admission, after baseline endoscopic biopsy (by flexible sigmoidoscopy), subjects are placed on a low fat, high fiber diet for 4 wks. Assessment of colonic epithelium, sodium butyrate, and Ki-ras oncogene mutations are performed using endoscopic biopsy, blood, and stool sample. SIGNIFICANCE OF STUDY: Colon cancer is a common human malignancy. Dietary fat and fiber have been shown to affect the incidence of colorectal cancer. Populations consuming diets high in fat and low in fiber overall have greater incidence rates of colorectal cancer, compared to populations consuming less fat and more fiber. It is not entirely clear whether dietary fat and fiber are independent variables with respect to colon carcinogenesis. Sodium butyrate, a short chain fatty acid, is generated in the colon in large quantities as dietary fiber is fermented by colonic bacteria. Sodium butyrate, in vitro, reduces the proliferation and induces apoptosis in cultured colon cancer cells. FUTURE PLANS: Once a sufficient subject population is studied and laboratory and statistical analyses are completed, we will be able to determine the effects of dietary fat and fiber on several parameters in the colonic mucosa which might be important indicators of colon cancer risk. Additional studies will focus on the level of fat and fiber that produces the highest risk for colon cancer and eventually to isolate those substances in this diet that are responsible for predisposition to colon cancer.