EFFECT OF FAT AND FIBER ON COLONIC INTESTINAL EPITHELIAL CELLS

这项研究考察了不同水平的饮食脂肪和 纤维对结肠上皮细胞增殖和死亡的影响。 受试者被给予良好控制的饮食(保持体重，代谢)， 脂肪/纤维组合(高脂肪、正常脂肪或低脂肪以及高、正常或 低纤维)作为自变量。入院时，基线后 内窥镜活检(通过柔性乙状结肠镜)，受试者被放置在 低脂肪、高纤维饮食4周。对结肠上皮的评估， 丁酸钠和Ki-ras癌基因突变使用 内窥镜活检、血液和粪便样本。研究意义：冒号 癌症是人类常见的恶性肿瘤。饮食中的脂肪和纤维 显示影响结直肠癌的发病率。消费人群 总体来说，高脂肪和低纤维饮食的发病率更高 结直肠癌，与摄入更少脂肪和更多脂肪的人群相比 纤维。目前还不完全清楚膳食脂肪和纤维是否 与结肠癌发生有关的独立变量。钠 丁酸是一种短链脂肪酸，在结肠中大量生成。 作为膳食纤维的数量是由结肠菌发酵的。钠 丁酸盐在体外抑制细胞增殖和诱导细胞凋亡 培养的结肠癌细胞。未来计划：曾经是一个足够的主题 研究了人口，实验室和统计分析是 完成后，我们将能够确定饮食脂肪和 结肠粘膜中纤维的几个参数，这可能是重要的 结肠癌风险的指标。其他研究将集中在 脂肪和纤维的水平，这会导致结肠癌和 最终分离出饮食中负责的那些物质 结肠癌的易感性。

This research examines the effect of different levels of dietary fat and fiber on the proliferation and death of colonic epithelial cells. Subjects are given a well-controlled diet (weight maintaining, metabolic), with fat/fiber combination (high, normal or low fat and high, normal or low fiber) as the independent variable. Upon admission, after baseline endoscopic biopsy (by flexible sigmoidoscopy), subjects are placed on a low fat, high fiber diet for 4 wks. Assessment of colonic epithelium, sodium butyrate, and Ki-ras oncogene mutations are performed using endoscopic biopsy, blood, and stool sample. SIGNIFICANCE OF STUDY: Colon cancer is a common human malignancy. Dietary fat and fiber have been shown to affect the incidence of colorectal cancer. Populations consuming diets high in fat and low in fiber overall have greater incidence rates of colorectal cancer, compared to populations consuming less fat and more fiber. It is not entirely clear whether dietary fat and fiber are independent variables with respect to colon carcinogenesis. Sodium butyrate, a short chain fatty acid, is generated in the colon in large quantities as dietary fiber is fermented by colonic bacteria. Sodium butyrate, in vitro, reduces the proliferation and induces apoptosis in cultured colon cancer cells. FUTURE PLANS: Once a sufficient subject population is studied and laboratory and statistical analyses are completed, we will be able to determine the effects of dietary fat and fiber on several parameters in the colonic mucosa which might be important indicators of colon cancer risk. Additional studies will focus on the level of fat and fiber that produces the highest risk for colon cancer and eventually to isolate those substances in this diet that are responsible for predisposition to colon cancer.