INDUCTION OF PLASMODIUM INFECTIONS TO SUPPORT MALARIA VACCINE STUDIES

诱导疟原虫感染以支持疟疾疫苗研究

• 批准号: 6277464
• 负责人: WILLIAM E COLLINS
• 金额: $ 5.38万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277464
• 关键词: Mammalia; Primates; biomaterials; hematology; human tissue; immunology; infection; inflammation; parasitism

Blood-stage parasites were obtained for (1) preparation of genomic libraries, (2) extraction of m-RNA for genetic engineering studies, (3) antigen for serologic tests, (4) infection of mosquitoes through membrane feeding to produce sporozoites for (a) genetic engineering studies, (b) production of monoclonal antibodies, and (c) to infect Aotus and Saimiri monkeys and to test the efficacy of experimental vaccines, and (5) production of immune sera. The following parasites and animals were inoculated during 1997 Plasmodium vivax - Conan (CO-521), Justin (C-529), and Luther (C-589); Plasmodium malariae - Keith (C-545), Marietta (C-472), Joseph (C-475), and Mason (C-515); Plasmodium ovale - Mason (C-515), and Suzanna (C-514).

获得血液期寄生虫用于（1）制备基因组 文库，（2）提取用于基因工程研究的m-RNA， (3)血清学试验用抗原，（4）通过 膜补料以产生用于（a）基因工程的子孢子 研究，（B）单克隆抗体的产生，和（c）感染 Aotus和Saimiri猴，并测试实验性 疫苗;（5）免疫血清的制备。 以下寄生虫 1997年间日疟原虫-科南 （CO-521）、Justin（C-529）和Luther（C-589）;三日疟原虫- 基思（C-545）、玛丽埃塔（C-472）、约瑟夫（C-475）和梅森（C-515）; 卵形疟原虫- Mason（C-515）和Suzanna（C-514）。