INDUCTION OF PLASMODIUM INFECTIONS TO SUPPORT MALARIA VACCINE STUDIES

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SPID#: 7 Short-term malarial infections were induced in chimpanzees to obtain blood-stage parasites for (1) preparation of genomic libraries, (2) extraction of m-RNA for genetic engineering studies, (3) antigen for serologic tests, (4) infection of mosquitoes through membrane feeding to produce sporozoites for (a) genetic engineering studies, (b) production of monoclonal antibodies, and (c) to infect Aotus and Saimiri monkeys and to test the efficacy of experimental vaccines, and (5) production of immune sera. The following malarial parasites and animals were inoculated during the past year Plasmodium ovale---animals CO-529 and CO-547; Plasmodium vivax----animals CO-547, CO-382, CO-529, CO-516, and CO-510. Three of the infections were induced via the bites of infected mosquitoes to circumvent the possibility of viral transmission. These studies will continue in support of the development of vaccines for human malarias.