DOWNREG LCK MEDIATED SIGNAL TRANSDUC BY Y114 MUTATION OF HERPESVIRUS SAIMIRI TIP

TIP与LCK的结合负性调节LCK介导 信号转导。疱疹病毒Simiri尖端与Lck相关 并下调LCK在细胞信号转导中的作用。在……里面 这份报告，我们证明了TIP的酪氨酸114的突变 显著提高LCK结合活性。这个突变体表现出一种 细胞酪氨酸抑制作用显著增强 淋巴细胞抗原的磷酸化和表面表达 与野生型Tip的比较。此外，TipY114的表达 转化成纤维细胞的转化形态 致癌的F505 Lck细胞形态正常。这些结果 进一步支持将TIP与LCK相关联的机制 负向调节LCK介导的信号转导。

The association of tip with lck negatively regulates Lck-mediated signal transduction. Tip of herpesvirus saimiri associates with Lck and downregulates Lck function in cellular signal transduction. In this report, we demonstrate that mutation of tyrosine 114 of Tip significantly increases Lck-binding activity. This mutant exhibits a dramatic increase in the suppression of cellular tyrosine phosphorylation and surface expression of lymphocyte antigens in comparison with wild-type Tip. In addition, the expression of TipY114 converted the transforming morphology of fibroblasts induced by oncogenic F505 Lck to a normal cellular morphology. These results further support a mechanism by which the association of Tip with Lck negatively regulates Lck-mediated signal transduction.