FROZEN HYDRATED STUDIES

冷冻水合研究

• 批准号: 6119912
• 负责人: Buddy D Ratner
• 金额: $ 6.8万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119912
• 关键词: animal tissue; bioengineering /biomedical engineering; biological products; biomaterials; biomedical equipment development; biomedical resource; biotechnology; proteins; technology /technique

Biomaterials placed in the body are exposed to an aqueous environment andtherefore exist, in practice, in a hydrated state. In order to apply UHV techniques such as SIMS and ESCA to such samples, it is necessary to employ cryogenic methods to ensure that the material retains its hydrated state. This project represents a continuation of our efforts to develop and implement reproducible cryogenic methods which would enable the use of these techniques on hydrated biomaterials. Plunge cooling and impact cooling methods are being investigated for protein and cell samples.

放置在体内的生物材料暴露在水溶液中