3 D TOMOGRAPHIC RECONSTRUCTION OF ISOLATED SPISULA CENTROSOMES

（部分由NIH GMS 40198支持。里德尔和NIH GMS 43264 于R. Palazzo）这是一个正在进行的项目，部分由RO 1提供支持 帕拉佐和里德尔博士的赠款金 此前，这些研究人员 描述了第一个中心粒/中心体的无细胞系统 复制的基础上的匀浆冲浪蛤卵母细胞。 由于中心体非常小（0.25 × 0.5 μ m）和复杂， 研究它们的形态和复制的唯一方法是 3D EM。 在过去的12年里，我们已经表明，HVEM的连续厚 这些研究极大地便利了这些研究。 我们正继续 合作，并将我们的研究扩展到三维断层扫描 重建。 共10个双倾斜断层扫描 使用IVEM从0.25 lm切片进行重建。 Vogel，J.M，S. Szarbor，C.L.里德尔和R. E.宫殿 1997 刺海鞘卵母细胞中心体含有环状结构 和不寻常的化学计量比（/（tublin. 细胞生物学杂志， 137：193-202。

(Supported in part by NIH GMS 40198 to C. Rieder and NIH GMS 43264 to R. Palazzo) This is an ongoing project supported, in part, by RO1 grants to Drs. Palazzo and Rieder. Previously these investigators described the first Cellular-free system for centriole/centrosome duplication which was based on homogenates of surf clam oocytes. Because centrosomes are extremely small (0.25 by 0.5 um) and complex, the only way their morphology and replication can be studied is with 3D EM. Over the past 12 years we have shown that HVEM of serial thick sections greatly facilitates such studies. We are continuing our collaboration and are extending our studies to 3-D tomographic reconstructions. A total of 10 double-tilt tomographic reconstructions were made from 0.25lm sections, using the IVEM. Vogel, J.M, S. Sterns, C.L. Rieder, and R. E. Palazzo. 1997 Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of (/( tublin. J. Cell Biol., 137:193-202.