3 D TOMOGRAPHIC RECONSTRUCTION OF ISOLATED SPISULA CENTROSOMES

(部分由NIH GMS 40198至C.Rieder和NIH GMS 43264支持 这是一个正在进行的项目，部分由RO1提供支持 帕拉佐博士和里德博士获奖。此前，这些调查人员 描述了第一个中心粒/中心体的无细胞系统 以SURF文蛤卵母细胞匀浆为基础的复制。 因为中心体非常小(0.25×0.5微米)和复杂， 研究它们的形态和复制的唯一方法是 3D EM。在过去的12年里，我们已经证明了 章节极大地便利了这类研究。我们正在继续我们的 并正在将我们的研究扩展到3D断层扫描 重建。共10次双倾斜断层扫描 采用IVEM对0.25lm切片进行重建。 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细内容九七 从Spisula solidissima卵母细胞分离的中心体含有环 和不寻常的化学计量比(/(tublin.J.Cell Biol. 137：193-202。

(Supported in part by NIH GMS 40198 to C. Rieder and NIH GMS 43264 to R. Palazzo) This is an ongoing project supported, in part, by RO1 grants to Drs. Palazzo and Rieder. Previously these investigators described the first Cellular-free system for centriole/centrosome duplication which was based on homogenates of surf clam oocytes. Because centrosomes are extremely small (0.25 by 0.5 um) and complex, the only way their morphology and replication can be studied is with 3D EM. Over the past 12 years we have shown that HVEM of serial thick sections greatly facilitates such studies. We are continuing our collaboration and are extending our studies to 3-D tomographic reconstructions. A total of 10 double-tilt tomographic reconstructions were made from 0.25lm sections, using the IVEM. Vogel, J.M, S. Sterns, C.L. Rieder, and R. E. Palazzo. 1997 Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of (/( tublin. J. Cell Biol., 137:193-202.