课题基金 / 基金详情

3 D TOMOGRAPHIC RECONSTRUCTION OF ISOLATED SPISULA CENTROSOMES

3 D TOMOGRAPHIC RECONSTRUCTION OF ISOLATED SPISULA CENTROSOMES
分离的 Spiula 中心体的 3D 断层扫描重建
批准号:
6280694
负责人:
ROBERT E PALAZZO
金额:
$1.08万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-01-01 至 1998-12-31

项目摘要

项目成果

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中文摘要
翻译
(部分由NIH GMS 40198向C. Rieder和NIH GMS 43264提供支持
英文摘要
(Supported in part by NIH GMS 40198 to C. Rieder and NIH GMS 43264 to R. Palazzo) This is an ongoing project supported, in part, by RO1 grants to Drs. Palazzo and Rieder. Previously these investigators described the first Cellular-free system for centriole/centrosome duplication which was based on homogenates of surf clam oocytes. Because centrosomes are extremely small (0.25 by 0.5 um) and complex, the only way their morphology and replication can be studied is with 3D EM. Over the past 12 years we have shown that HVEM of serial thick sections greatly facilitates such studies. We are continuing our collaboration and are extending our studies to 3-D tomographic reconstructions. A total of 10 double-tilt tomographic reconstructions were made from 0.25lm sections, using the IVEM. Vogel, J.M, S. Sterns, C.L. Rieder, and R. E. Palazzo. 1997 Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of (/( tublin. J. Cell Biol., 137:193-202.
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CENTROSOME STRUCTURE
  • 批准号:
    7598366
  • 项目类别:
  • 资助金额:
    $2.29万
  • 财政年份:
    2007
  • 负责人:
    ROBERT E PALAZZO
  • 依托单位:
IVEM TOMOGRAPHY OF ISOLATED EXTRACTED & RE CONSTRUCTED SPISULA CENTROSOMES: CLAM
  • 批准号:
    6653382
  • 项目类别:
  • 资助金额:
    $29.46万
  • 财政年份:
    2002
  • 负责人:
    ROBERT E PALAZZO
  • 依托单位:
IVEM TOMOGRAPHY OF ISOLATED EXTRACTED & RE CONSTRUCTED SPISULA CENTROSOMES: CLAM
  • 批准号:
    6491865
  • 项目类别:
  • 资助金额:
    $29.46万
  • 财政年份:
    2001
  • 负责人:
    ROBERT E PALAZZO
  • 依托单位:
IVEM TOMOGRAPHY OF ISOLATED EXTRACTED & RE CONSTRUCTED SPISULA CENTROSOMES: CLAM
  • 批准号:
    6423448
  • 项目类别:
  • 资助金额:
    $29.46万
  • 财政年份:
    2000
  • 负责人:
    ROBERT E PALAZZO
  • 依托单位:
海外基金