SYNCHROTRON CRYSTALLOGRAPHY OF RANTES

RANTES 的同步加速器晶体学

• 批准号: 6120418
• 负责人: ALEX WLODAWER
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120418
• 关键词: biological products; biomedical resource; enzymes; spectrometry

A small crystal of AOP-RANTES (0.1 X 0.05 X 0.05 mm3) was rapidly cooled in the nitrogen stream and diffracted to 1.6 A resolution in 30 sec exposures. 159 frames of 1 degree oscillation were collected at 0.98 A wavelength. This resulted in a complete data set of excellent quality from 20 to 1.6 A resolution, with 99.8% overall completeness, I/sigma(I) of 31.8, and 11-fold redundancy. The structure was solved by molecular replacement, using the structure of Met-RANTES as a search model, and has been refined with the final R-factor of 16.7%, and a final R-free of 24.1%.

AOP-RANTES 的小晶体 (0.1 X 0.05 X 0.05 mm3) 迅速 在氮气流中冷却并在 30 秒内衍射至 1.6 A 分辨率 秒曝光。 收集了 159 帧 1 度振荡 0.98 A 波长。 这产生了一个完整的优秀数据集 质量分辨率从 20 A 到 1.6 A，整体完整性为 99.8%， I/sigma(I) 为 31.8，冗余度为 11 倍。 结构已经解决了 通过分子置换，使用 Met-RANTES 的结构作为 搜索模型，并经过细化，最终 R 因子为 16.7%， 最终的无 R 率为 24.1%。