CONSTRUCT BEAMLINE X 9A RESOURCE DEDICATED TO TIME RESOLVED XRAY FOOTPRINTING

构建专用于时间分辨 X 射线足迹的 Beamline X 9A 资源

• 批准号: 6120332
• 负责人: MARK R CHANCE
• 金额: $ 4.04万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120332
• 关键词: biomedical equipment development; biomedical resource; biotechnology; building /facility design /renovation; nucleic acids; proteins; radiology; spectrometry; technology /technique

To provide dedicated access to footprinting technology, beamline X9A at the National Synchrotron Light Source has been constructed along with a dedicated stopped flow instrument to allow footprinting studies for our expanded group of investigators. The beamline accepts up to 5 milliradians of white beam, with a flux density of 109 photons/s/mm2/0.07% bandwidth over an effective energy range of 3-30 keV. The completion of the beamline has enhanced the productivity of this core project, accelerating both the core and collaborative research programs tremendously. Our Resource is the world leader in this technology and the availability of a dedicated beamline will continue our leadership in this area. We are now routinely conducting Hfootprinting studies on 10 - 20 msec and longer timescales with our Hstopped flow device. Major advances in understanding RNA folding have Hbeen accomplished and studies of protein-DNA complex formation have Hbeen initiated.

为了提供足迹技术的专用通道， 国家同步辐射光源X9 A已建成 沿着使用专用停流仪器，以允许足迹 为我们扩大的研究小组进行研究。 光束线接受 高达5毫弧度的白色光束，通量密度为109 光子/s/mm 2/0.07%带宽，有效能量范围为3-30 凯 光束线的建成提高了 这个核心项目，加速核心和协作 研究项目非常多。 我们的资源是世界领先的 这种技术和专用光束线的可用性将 我们将继续在这一领域发挥领导作用。 我们现在例行地进行 使用我们的方法对10 - 20毫秒和更长时间尺度的足迹研究 停止流动装置。 理解RNA折叠的主要进展 蛋白质-DNA复合物形成的研究 已经开始了。