SUBCELL LOCALIZATION OF BPD MA IN ADHERENT CELLS UTILIZING TWO PHOTON MICROSCOPY
SUBCELL LOCALIZATION OF BPD MA IN ADHERENT CELLS UTILIZING TWO PHOTON MICROSCOPY
批准号:
6121907
负责人:
MICHAEL G NICHOLS
金额:
$0.22万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-30 至 1999-08-31
中文摘要
我们建议评估BPD-MA的双光子显微镜来研究
在各种条件下的亚细胞定位。
苯并卟啉衍生物是第二代光敏剂
目前正在进行第二阶段临床试验的化合物用于
治疗银屑病、老年性黄斑变性以及
皮肤肿瘤学。此前的研究表明,这种药物
是一种有效的增敏剂,其杀灭细胞的功效在
浓度低于200 ng/ml。它的主要亚细胞靶点是
被认为是线粒体,在那里已经被证明
BPD-MA可以光动力解偶联细胞内的氧化磷酸化
分离大鼠线粒体。我们最初的实验试图
优化双光子显微镜用于该药物的观察。这个
BPD-MA在脂质体中的双光子散射截面
最近测量的波长范围为820-980 nm。量了十字之后
部分中,我们将能够评估检测阈值
然后在贴壁细胞培养中培养。细胞器定位
已概述的研究工作预计将在不久的将来继续进行。
英文摘要
We propose to evaluate two-photon microscopy of BPD-MA to study
subcellular localization under a variety of conditions.
Benzoporphyrin Derivative is a second-generation photosensitizing
compound currently undergoing phase II clinical trials for use in
treating psoriasis, age-related macular degeneration as well as
cutaneous oncology. Previous research has demonstrated that this drug
is a potent sensitizer, with efficacy in cell killing demonstrated at
concentrations below 200 ng/ml. Its primary subcellular target is
believed to the mitochondria, where it has been demonstrated that
BPD-MA can photodynamically uncouple oxidative phosphorylation in the
isolated rat mitochondria. Our initial experiments have sought to
optimize two-photon microscopy for observation of this drug. The
two-photon cross section of BPD-MA in a liposomal preparation has
recently been measured from 820-980 nm. Having measured the cross
section, we will be able to evaluate a detection threshold that will
then be ve rified in adherent cell culture. Organelle localization
studies have been outlined are expected to proceed in the near future.
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会议论文
ASSESSMENT OF CELLULAR ENERGETICS BY NADH FLIM
-
批准号:8360017
-
项目类别:
-
资助金额:$1.43万
-
财政年份:2011
-
负责人:MICHAEL G NICHOLS
-
依托单位:
ASSESSMENT OF CELLULAR ENERGETICS BY NADH FLIM
-
批准号:8167504
-
项目类别:
-
资助金额:$1.44万
-
财政年份:2010
-
负责人:MICHAEL G NICHOLS
-
依托单位:
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
-
批准号:7960280
-
项目类别:
-
资助金额:$1.32万
-
财政年份:2009
-
负责人:MICHAEL G NICHOLS
-
依托单位:
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
-
批准号:7725204
-
项目类别:
-
资助金额:$1.47万
-
财政年份:2008
-
负责人:MICHAEL G NICHOLS
-
依托单位:
Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
-
批准号:7516180
-
项目类别:
-
资助金额:$21.68万
-
财政年份:2008
-
负责人:MICHAEL G NICHOLS
-
依托单位:
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
-
批准号:7627629
-
项目类别:
-
资助金额:$1.42万
-
财政年份:2007
-
负责人:MICHAEL G NICHOLS
-
依托单位:
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
-
批准号:7381544
-
项目类别:
-
资助金额:$1.46万
-
财政年份:2006
-
负责人:MICHAEL G NICHOLS
-
依托单位:
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
-
批准号:7170770
-
项目类别:
-
资助金额:$1.65万
-
财政年份:2005
-
负责人:MICHAEL G NICHOLS
-
依托单位:
BLEACHING KINETICS OF NADH IN SOLUTION
-
批准号:6121886
-
项目类别:
-
资助金额:$1.12万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
INVITED TALK BIOPHYSICAL IMAGING W/ MULTIPHOTON MICROSCOPY
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批准号:6121967
-
项目类别:
-
资助金额:$0.07万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
OBSERVE CHANGES IN CELLULAR AUTOFLUORESCENCE DURING EARLY STAGES OF APOPTOSIS
-
批准号:6121885
-
项目类别:
-
资助金额:$1.57万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
SETUP & APPL OF MULTIPHOTON MICROSCOPE: IMAGE DRUG DISTRIBUTION IN HUMAN SKIN
-
批准号:6121966
-
项目类别:
-
资助金额:$0.01万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
IMAGE NADH AUTOFLUORESCENCE IN MULTICELL TUMOR SPHEROIDS & MONOLAYER CULTURE
-
批准号:6121883
-
项目类别:
-
资助金额:$3.59万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
IMAGE NADH AUTOFLUORESCENCE DURING PDT; PHOTOFRIN & PDT ON CELLULAR AUTOFLUORES
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批准号:6121884
-
项目类别:
-
资助金额:$3.59万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
TALK: IMAGE PHOTOFRIN & NADH AUTOFLUORESCENCE IN CELL MONOLAYER & TUMOR SPHEROID
-
批准号:6121968
-
项目类别:
-
资助金额:$0.07万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
TWO PHOTON IMAGING OF ALA INDUCED PPIX IN NORMAL & CANCER CELLS & TISSUES
-
批准号:6121908
-
项目类别:
-
资助金额:$0.11万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
TWO PHOTON IMAGING OF MOUSE OOCYTES & EMBRYOS
-
批准号:6121909
-
项目类别:
-
资助金额:$0.14万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
MEASUREMENTS OF MULTIPHOTON CROSS SECTIONS FOR SEVERAL PHOTOSENSITIZERS
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批准号:6121887
-
项目类别:
-
资助金额:$1.36万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
TWO-PHOTON IMAGING OF NAD(P)H IN TUMOR SPHEROIDS
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批准号:2769894
-
项目类别:
-
资助金额:$2.92万
-
财政年份:1998
-
负责人:MICHAEL G NICHOLS
-
依托单位:
SURVEY OF MULTIPHOTON EXCITED AUTOFLUORESCENCE IN HELA & EMI~ & RO CELL LINES
-
批准号:6252951
-
项目类别:
-
资助金额:$0.81万
-
财政年份:1997
-
负责人:MICHAEL G NICHOLS
-
依托单位: