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SUBCELL LOCALIZATION OF BPD MA IN ADHERENT CELLS UTILIZING TWO PHOTON MICROSCOPY

SUBCELL LOCALIZATION OF BPD MA IN ADHERENT CELLS UTILIZING TWO PHOTON MICROSCOPY
利用两个光子显微镜对贴壁细胞中的 BPD MA 进行亚细胞定位
批准号:
6121907
负责人:
MICHAEL G NICHOLS
金额:
$0.22万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-30 至 1999-08-31

项目摘要

项目成果

MICHAEL G NICHOLS的其他基金

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中文摘要
翻译
我们建议利用双光子显微镜对BPD-MA进行研究
英文摘要
We propose to evaluate two-photon microscopy of BPD-MA to study subcellular localization under a variety of conditions. Benzoporphyrin Derivative is a second-generation photosensitizing compound currently undergoing phase II clinical trials for use in treating psoriasis, age-related macular degeneration as well as cutaneous oncology. Previous research has demonstrated that this drug is a potent sensitizer, with efficacy in cell killing demonstrated at concentrations below 200 ng/ml. Its primary subcellular target is believed to the mitochondria, where it has been demonstrated that BPD-MA can photodynamically uncouple oxidative phosphorylation in the isolated rat mitochondria. Our initial experiments have sought to optimize two-photon microscopy for observation of this drug. The two-photon cross section of BPD-MA in a liposomal preparation has recently been measured from 820-980 nm. Having measured the cross section, we will be able to evaluate a detection threshold that will then be ve rified in adherent cell culture. Organelle localization studies have been outlined are expected to proceed in the near future.
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