TWO PHOTON IMAGING OF ALA INDUCED PPIX IN NORMAL & CANCER CELLS & TISSUES

正常情况下 ALA 诱导的 PPIX 的两次光子成像

• 批准号: 6121908
• 负责人: MICHAEL G NICHOLS
• 金额: $ 0.11万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121908
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical resource; human tissue; lasers; skin; technology /technique

Our goals are to acquire simultaneous two-photon images of ALA-induced PpIX and NADH autofluorescence using several well-defined cell lines and normal and tumor tissues of the balb-c mouse. While much is known regarding the heme biosynthesis pathway, recent steady-state fluorescence polarization studies indicate the possibility that the site of PpIX formation may differ in normal and tumor tissues. We propose to address this issue using multiphoton excited NADH autofluorescence and possibly other extrinsic mitochondrial indicators to observe the localization of ALA-induced PpIX in the samples cited above.

我们的目标是获得同时的双光子图像 ALA诱导的PpIX和NADH自体荧光 Balb-c小鼠的细胞系、正常组织和肿瘤组织。而当 关于血红素的生物合成途径，人们知道的很多，最近 稳态荧光偏振研究表明 PPIX形成的位置可能在正常情况下和在 肿瘤组织。我们建议使用多光子来解决这个问题 激发的NADH自身荧光和可能的其他外在因素 用线粒体指示剂观察ALA诱导的细胞定位 上面引用的样品中的PpIX。