INVITED TALK BIOPHYSICAL IMAGING W/ MULTIPHOTON MICROSCOPY

多光子显微镜生物物理成像特邀演讲

• 批准号: 6121967
• 负责人: MICHAEL G NICHOLS
• 金额: $ 0.07万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121967
• 关键词: bioengineering /biomedical engineering; biomedical resource; education; technology /technique

46th annual meeting of the Radiation Research Society Conference, Louisville, KY Optical imaging of living cells and intact tissues by non-linear, simultaneous multiphoton molecular excitation provides inherent three-dimensional diffraction-limited resolution while minimizing photodamage and peripheral photobleaching. The multiphoton laser scanning microscope raster-scans a focused, high repetition-rate femtosecond pulse train of infrared laser light across biological samples and efficiently detects the resulting multiphoton excited fluorescence. This development has recently allowed quantitative visualization of cellular processes and tissue architecture that had been problematic or impossible with conventional microscopies. The physical principles, aspects of instrumentation developments, and photophysical characterization of molecular fluorophores that have enabled multiphoton microscopy will be discussed. Examples such as neurotransmitter secretion, skin morphology, drug metabolism in the intact colon, and simultaneous metabolic and photosensitizer imaging in t umor spheroids demonstrate a range of applications pursued with this novel form of microscopy at the Developmental Resource for Biophysical Imaging and Opto-electronics (DRBIO) at Cornell. Supported at DRBIO by the NIH RR04224. M.G.N. is an NIH Postdoctoral Fellow supported by NRSA 1 F32 CA 72225-02.

第46届辐射研究学会年会， Louisville，KY活细胞和完整组织的光学成像 非线性的同时多光子分子激发提供了 固有三维衍射极限分辨率， 最大限度地减少光损伤和外周光漂白。 多光子 激光扫描显微镜光栅扫描聚焦，高重复率 飞秒红外激光脉冲序列对生物组织的影响 采样并有效地检测所产生的多光子激发 荧光。 这一发展最近允许定量 细胞过程和组织结构的可视化， 用常规显微镜是有问题的或不可能的。 的 物理原理，仪器开发方面，以及 分子荧光团的生物物理表征， 启用多光子显微镜将被讨论。 例子如 神经递质分泌，皮肤形态，药物代谢， 完整结肠，同时代谢和光敏剂成像 在肿瘤中，球状体显示了一系列应用， 这种新形式的显微镜在发展资源， 生物物理成像和光电（DRBIO）在康奈尔大学。 DRBIO由NIH RR 04224支持。 M.G. N是NIH博士后 由NRSA 1 F32 CA 72225-02支持的研究员。