课题基金 / 基金详情

TWO PHOTON IMAGING OF MOUSE OOCYTES & EMBRYOS

TWO PHOTON IMAGING OF MOUSE OOCYTES & EMBRYOS
小鼠卵母细胞的两次光子成像
批准号：
6121909
负责人：
MICHAEL G NICHOLS
金额：
$0.14万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-09-30 至 1999-08-31
来源：
https://reporter.nih.gov/project-details/6121909
关键词：
animal tissue bioengineering /biomedical engineering biomedical resource growth /development human tissue lasers reproductive system technology /technique

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英文摘要
Our long-range goal is to be able to non-invasively distinguish viable from non-viable embryos by image analysis. As a starting point we propose to use NADH imaging to visualize mitochondrial distribution in the embryos. There is recent evidence that in bovine oocytes stained with Mitotracker Rosamine the mitochondrial distribution is different in oocytes cultured in optimal versus suboptimal media. Initial experiments at the resource have demonstrated that NADH autofluorescence indeed correlates with the Mitotracker signal in freshly excised mouse oocytes. Futhermore, differences in morphology between oocytes is easily observed via two-photon excited NADH autofluorescence. A systematic study of oocyte health as reported by cellular autofluorescence is planned in the near future. 1 DLPC - dilauroyl-sn-glycero-3-phosphocholine, DPPC - dipalmitoyl-sn-glycero-3-phosphocholine, DMPC - dimyristoyl-sn-glycero-3-phosphocholine,
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ASSESSMENT OF CELLULAR ENERGETICS BY NADH FLIM
ASSESSMENT OF CELLULAR ENERGETICS BY NADH FLIM
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING
RESPONSE OF OSTEOGENIC CELLS TO OPTICAL STRETCHING