2 PHOTON ABSORPTION

2 光子吸收

• 批准号: 6121629
• 负责人: NICHOLAS BARRY
• 金额: $ 0.19万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121629
• 关键词: biomedical resource; spectrometry; technology /technique

We are developing an intrument for the measurement of the 2-photon absoption cross-sections, of biological releint fluorepohores over a wide wavelenght range. The excitation source is a broadly tunable (700nm - 1 000nm) modelocked Ti-sapphire laser. Excitation into the visible range is possible using a modelocked dye laser. To avoid intensity errors due to pre-dispersed of the ultra short laser pulses in the instruments optics, the laser pulses are predispessed to a duration of about 1 pico second. By moldulating the excitation light we are also able to distinguish between two and three photon excitation effects.

我们正在研制一种双光子测量仪器 吸收截面，生物荧光团在 宽波长范围。 激发源是一个广泛可调的 （700 nm- 1 000 nm）锁模钛蓝宝石激光器。 激励进入 使用锁模染料激光器可以实现可见范围。 避免 由于超短激光脉冲的预分散引起的强度误差 在仪器的光学系统中，激光脉冲被预先分配到 持续时间约为1皮科。 通过调制激发光 我们也能够区分两个和三个光子 激励效应