CONFOCAL RAMAN MICROSPECTROSCOPY FOR BIOLOGICAL MICROSTRUCTURE ANALYSIS

用于生物微观结构分析的共焦拉曼显微镜

• 批准号: 6281766
• 负责人: GEURT DEINUM
• 金额: $ 1.89万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281766
• 关键词: biomedical equipment development; biomedical resource; cardiovascular system; clinical research; human tissue; microscopy; spectrometry; technology /technique; women's health

We have augmented our bulk near IR Raman spectroscopy with confocal microscope illumination and collection of Raman signals from thin sections with very high spatial resolution on the order of 1 micron. The novel optical design enables switching between bulk and microscope spectroscopic studies fairly easy. We have used this confocal Raman microscope system for collecting spectra of a variety of morphological structures in artery, breast, and brain tissues. The ultimate goal is to use these information for the understanding of microscopic and morphological basis of Raman signals from bulk tissues and correlate with histopathology.

我们增强了我们的近红外拉曼光谱与 共焦显微镜照明和拉曼信号的收集， 薄切片具有非常高的空间分辨率，约为1 微米。 新颖的光学设计使得能够在批量和 显微镜光谱研究相当容易。 我们已经用这种 用于收集多种光谱的共焦拉曼显微镜系统 动脉、乳腺和脑组织的形态结构。 的 最终目标是利用这些信息来理解 来自大块组织的拉曼信号的微观和形态学基础 并与组织病理学相关联。