FLUORESCENCE DETECTION OF PAPILLARY TUMOR AT CYSTECTOMY

膀胱切除术中乳头状肿瘤的荧光检测

• 批准号: 6281788
• 负责人: JOSEPH T ARENDT
• 金额: $ 0.76万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281788
• 关键词: bioengineering /biomedical engineering; biomedical resource; human tissue; model design /development; statistics /biometry; urinary tract

The goal of the cystectomy study is to use autofluorescence spectroscopy to diagnose normal mucosa from dysplasia, carcinoma-in-situ, invasive cancer, and papillary tumors in bladder. Principal component analysis (PCA) followed by least-squares regression (LR) was applied to the data set. For 27 patients taken with 400 nm excitation, a calibration set and a validation set were randomly formed with 13 and 14 patients respectively. With an imposed limitation of three categories of normal mucosa, CIS, and invasive cancer, an attempt with PCA and LR on the validation set produced 60% detection of invasive cancer, 0.8% of CIS, and 91.8% of normal mucosa. Changing the categories to normal vs. disease, with the disease category including CIS and invasive cancer, detected 86.7% of disease and 55.8% of normal mucosa. However, hemorrhage, inflammation, and other factors were not accounted for, yet later investigation showed hemorrhage decreased signal intensity by an average of around 25% and severe inflammation by an average of around 50%. Accounting for these effects is now ongoing. For 29 patients with 370 nm excitation, a calibration set and a validation set were randomly formed with 14 and 15 patients respectively, but these are also in the process of being redone to account for the effects of hemorrhage, inflammation, and edema.

膀胱切除术研究的目标是使用自体荧光 光谱学诊断正常粘膜与不典型增生， 膀胱原位癌、浸润癌和乳头状癌。 主成分分析(PCA)和最小二乘 对数据集进行回归分析(LR)。27名患者服用 在400 nm激发下，建立了校准组和验证组 随机分为13例和14例。用一种强加的 正常黏膜、顺应性黏膜和侵袭性黏膜三种类型的局限性 癌症，在验证集上尝试使用PCA和LR产生了60% 浸润性癌的检出率为0.8%，正常粘膜的检出率为91.8%。 将类别更改为正常与疾病，以及疾病 包括CIS和浸润性癌症在内的类别，发现了86.7%的疾病 正常粘膜占55.8%。然而，出血、炎症和 其他因素没有被考虑在内，但后来的调查显示 出血使信号强度平均降低约25%， 严重炎症的比例平均在50%左右。考虑到这些因素 影响现在正在进行中。对于29名370 nm激发的患者，a 校准组和验证组由14和 分别为15名患者，但这些患者也正在 重做以考虑出血、炎症和 浮肿。